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Université de Fribourg

An inflamed human alveolar model for testing the efficiency of anti-inflammatory drugs in vitro

Drasler, Barbara ; Karakocak, Bedia Begum ; Tankus, Esma Bahar ; Barosova, Hana ; Abe, Jun ; Sousa de Almeida, Mauro ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Frontiers in Bioengineering and Biotechnology, 2020, vol. 8, p. -

A large number of prevalent lung diseases is associated with tissue inflammation. Clinically, corticosteroid therapies are applied systemically or via inhalation for the treatment of lung inflammation, and a number of novel therapies are being developed that require preclinical testing. In alveoli, macrophages and dendritic cells play a key role in initiating and diminishing pro-inflammatory...

Università della Svizzera italiana

IL-12 protects from psoriasiform skin inflammation

Kulig, Paulina ; Musiol, Stephanie ; Freiberger, Sandra Nicole ; Schreiner, Bettina ; Gyülveszi, Gabor ; Russo, Giancarlo ; Pantelyushin, Stanislav ; Kishihara, Kenji ; Alessandrini, Francesca ; Kündig, Thomas ; Sallusto, Federica ; Hofbauer, Günther F.L. ; Haak, Stefan ; Becher, Burkhard

In: Nature communications, 2016, vol. 7, p. 13466

Neutralization of the common p40-subunit of IL-12/23 in psoriasis patients has led to a breakthrough in the management of moderate to severe disease. Aside from neutralizing IL-23, which is thought to be responsible for the curative effect, anti-p40 therapy also interferes with IL-12 signalling and type 1 immunity. Here we dissect the individual contribution of these two cytokines to the...

Università della Svizzera italiana

Memory T cells in latent mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset

Lindestam Arlehamn, Cecilia S. ; Gerasimova, Anna ; Mele, Federico ; Henderson, Ryan ; Swann, Justine ; Greenbaum, Jason A. ; Kim, Yohan ; Sidney, John ; James, Eddie A. ; Taplitz, Randy ; McKinney, Denise M. ; Kwok, William W. ; Grey, Howard ; Sallusto, Federica ; Peters, Bjoern ; Sette, Alessandro

In: Plos pathogens, 2013, vol. 9, no. 1, p. e1003130

An understanding of the immunological footprint of Mycobacterium tuberculosis (MTB) CD4 T cell recognition is still incomplete. Here we report that human Th1 cells specific for MTB are largely contained in a CXCR3+CCR6+ memory subset and highly focused on three broadly immunodominant antigenic islands, all related to bacterial secretion systems. Our results refute the notion that secreted...

Università della Svizzera italiana

Activin-A co-opts IRF4 and AhR signaling to induce human regulatory T cells that restrain asthmatic responses

Tousa, Sofia ; Semitekolou, Maria ; Morianos, Ioannis ; Banos, Aggelos ; Trochoutsou, Aikaterini I. ; Brodie, Tess M. ; Poulos, Nikolaos ; Samitas, Konstantinos ; Kapasa, Maria ; Konstantopoulos, Dimitris ; Paraskevopoulos, Giannis ; Gaga, Mina ; Hawrylowicz, Catherine M. ; Sallusto, Federica ; Xanthou, Georgina

In: Proceedings of the national academy of sciences of the United States of America, 2017, vol. 114, no. 14, p. E2891-E2900

Type 1 regulatory T (Tr1) cells play a pivotal role in restraining human T-cell responses toward environmental allergens and protecting against allergic diseases. Still, the precise molecular cues that underlie their transcriptional and functional specification remain elusive. Here, we show that the cytokine activin-A instructs the generation of CD4+ T cells that express the...

Université de Fribourg

Characterization and in vivo validation of a three-dimensional multi-cellular culture model to study heterotypic interactions in colorectal cancer cell growth, invasion and metastasis

Cattin, Sarah ; Ramont, Laurent ; Rüegg, Curzio

In: Frontiers in Bioengineering and Biotechnology, 2018, vol. 6, p. -

Colorectal cancer (CRC) is the third cause of cancer-related mortality in industrialized countries. Local invasion and metastasis formation are events associated with poor prognosis for which today there are no effective therapeutic options. Invasion and metastasis are strongly modulated by cells of the tumor microenvironment (TME), in particular fibroblasts and endothelial cells. Unraveling...