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Université de Fribourg

Skin proteomics – analysis of the extracellular matrix in health and disease

Dengjel, Jörn ; Bruckner-Tuderman, Leena ; Nyström, Alexander

In: Expert Review of Proteomics, 2020, vol. 17, no. 5, p. 377–391

Introduction The skin protects the human body from external insults and regulates water and temperature homeostasis. A highly developed extracellular matrix (ECM) supports the skin and instructs its cell functions. Reduced functionality of the ECM is often associated with skin diseases that cause physical impairment and also have implications on social interactions and quality of life of...

Université de Fribourg

Proteomic profiling of fibroblasts isolated from chronic wounds identifies disease-relevant signaling pathways

Berberich, Bettina ; Thriene, Kerstin ; Gretzmeier, Christine ; Kühl, Tobias ; Bayer, Hans ; Athanasiou, Ioannis ; Rafei-Shamsabadi, David Ali ; Bruckner-Tuderman, Leena ; Nyström, Alexander ; Kiritsi, Dimitra ; Dengjel, Jörn

In: Journal of Investigative Dermatology, 2020, vol. 140, no. 11, p. 2280-2290.e4

Chronic skin wounds accompany many prevalent age-related diseases and are a major cause of morbidity and mortality. Both keratinocytes and fibroblasts contribute to the pathomechanisms in chronic skin wounds. Dysregulated pathways in the epidermis have been extensively studied, but little is known of the influence of dermal fibroblasts on chronic wounding. We isolated fibroblasts from chronic...

Università della Svizzera italiana

Identification of a novel non-desmoglein autoantigen in Pemphigus Vulgaris

Di Lullo, Giulia ; Calabresi, Valentina ; Mariotti, Feliciana ; Zambruno, Giovanna ; Lanzavecchia, Antonio ; Di Zenzo, Giovanni

In: Frontiers in immunology, 2019, vol. 10, p. 1391

Pemphigus vulgaris (PV) is an autoimmune bullous disease of the skin and mucous membranes characterized by the presence of circulating and tissue-bound autoantibodies against keratinocyte cell surface antigens, specifically desmoglein (Dsg) 1 and 3. The pathogenic role of anti-Dsg antibodies is well-established, while the mechanism of blister formation is only partly defined. We have applied...

Università della Svizzera italiana

IL-12 protects from psoriasiform skin inflammation

Kulig, Paulina ; Musiol, Stephanie ; Freiberger, Sandra Nicole ; Schreiner, Bettina ; Gyülveszi, Gabor ; Russo, Giancarlo ; Pantelyushin, Stanislav ; Kishihara, Kenji ; Alessandrini, Francesca ; Kündig, Thomas ; Sallusto, Federica ; Hofbauer, Günther F.L. ; Haak, Stefan ; Becher, Burkhard

In: Nature communications, 2016, vol. 7, p. 13466

Neutralization of the common p40-subunit of IL-12/23 in psoriasis patients has led to a breakthrough in the management of moderate to severe disease. Aside from neutralizing IL-23, which is thought to be responsible for the curative effect, anti-p40 therapy also interferes with IL-12 signalling and type 1 immunity. Here we dissect the individual contribution of these two cytokines to the...

Université de Fribourg

Treatment of keratinocytes with 4-phenylbutyrate in epidermolysis bullosa: Lessons for therapies in keratin disorders

Spörrer, Marina ; Prochnicki, Ania ; C.Tölle, Regine ; Nyström, Alexander ; Esser, Philipp R. ; Homberg, Melanie ; Athanasiou, Ioannis ; Zingkou, Eleni ; Schillinga, Achim ; Gerum, Richard ; Thievessen, Ingo ; Winter, Lilli ; Bruckner-Tuderman, Leena ; Fabry, Ben ; Magin, Thomas M. ; Dengjel, Jörn ; Schröder, Rolf ; Kiritsi, Dimitra

In: EBioMedicine, 2019, vol. 44, p. 502–515

Missense mutations in keratin 5 and 14 genes cause the severe skin fragility disorder epidermolysis bullosa simplex (EBS) by collapsing of the keratin cytoskeleton into cytoplasmic protein aggregates. Despite intense efforts, no molecular therapies are available, mostly due to the complex phenotype of EBS, comprising cell fragility, diminished adhesion, skin inflammation and itch.Methods: We...