Université de Fribourg

Pathogenicity genomic island-associated CrpP-like fluoroquinolone-modifying enzymes among Pseudomonas aeruginosa clinical isolates in Europe

Ortiz de la Rosa, José Manuel ; Nordmann, Patrice ; Poirel, Laurent

In: Antimicrobial Agents and Chemotherapy, 2020, vol. 64, no. 7, p. -

Many transferable quinolone resistance mechanisms have been identified in Gram- negative bacteria. The plasmid-encoded 65-amino-acid-long ciprofloxacin-modifying enzyme CrpP was recently identified in Pseudomonas aeruginosa isolates. We analyzed a collection of 100 clonally unrelated and multidrug-resistant P. aeruginosa clinical isolates, among which 46 were positive for crpP-like genes,...

Université de Fribourg

Antimicrobial Resistance in Escherichia coli

Poirel, Laurent ; Madec, Jean-Yves ; Lupo, Agnese ; Schink, Anne-Kathrin ; Kieffer, Nicolas ; Nordmann, Patrice ; Schwarz, Stefan

In: Microbiology Spectrum, 2018, vol. 6, no. 4, p. -

Multidrug resistance in Escherichia coli has become a worrying issue that is increasingly observed in human but also in veterinary medicine worldwide. E. coli is intrinsically susceptible to almost all clinically relevant antimicrobial agents, but this bacterial species has a great capacity to accumulate resistance genes, mostly through horizontal gene transfer. The most problematic...

Université de Fribourg

Evaluation of the Rapid Polymyxin NP test and its industrial version for the detection of polymyxin-resistant Enterobacteriaceae

Jayol, Aurélie ; Kieffer, Nicolas ; Poirel, Laurent ; Guérind, François ; Güneser, Deniz ; Cattoir, Vincent ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2018, vol. 92, no. 2, p. 90–94

The commercial Rapid Polymyxin NP test was evaluated to detect colistin-resistant Enterobacteriaceae. A total of 223 enterobacterial isolates corresponding to 136 resistant (including 38 MCR-like producers), 19 heteroresistant, and 78 colistin- susceptible isolates were tested. The test was performed according to the manufacturer's instruction, and the color of the wells was read after 2 and 3...