Università della Svizzera italiana

Influenza hemagglutinin membrane anchor

Benton, Donald J. ; Nans, Andrea ; Calder, Lesley J. ; Turner, Jack ; Neu, Ursula ; Lin, Yi Pu ; Ketelaars, Esther ; Kallewaard, Nicole L. ; Corti, Davide ; Lanzavecchia, Antonio ; Gamblin, Steven J. ; Rosenthal, Peter B. ; Skehel, John J.

In: Proceedings of the national academy of sciences of the United States of America, 2018, vol. 115, no. 40, p. 10112-10117

Viruses with membranes fuse them with cellular membranes, to transfer their genomes into cells at the beginning of infection. For Influenza virus, the membrane glycoprotein involved in fusion is the hemagglutinin (HA), the 3D structure of which is known from X-ray crystallographic studies. The soluble ectodomain fragments used in these studies lacked the “membrane anchor” portion of the...

Université de Fribourg

Ecdysone triggered PGRP‐LC expression controls Drosophila innate immunity

Rus, Florentina ; Flatt, Thomas ; Tong, Mei ; Aggarwal, Kamna ; Okuda, Kendi ; Kleino, Anni ; Yates, Elisabeth ; Tatar, Marc ; Silverman, Neal

In: The EMBO Journal, 2013, vol. 32, no. 11, p. 1626–1638

Throughout the animal kingdom, steroid hormones have been implicated in the defense against microbial infection, but how these systemic signals control immunity is unclear. Here, we show that the steroid hormone ecdysone controls the expression of the pattern recognition receptor PGRP‐LC in Drosophila, thereby tightly regulating innate immune recognition and defense against bacterial...

Università della Svizzera italiana

Imaging cell interaction in tracheal mucosa during influenza virus infection using two-photon intravital microscopy

Palomino-Segura, Miguel ; Virgilio, Tommaso ; Morone, Diego ; Pizzagalli, Diego U. ; Gonzalez, Santiago F.

In: Journal of visualized experiments, 2018, vol. 138, p. e58355

The analysis of cell-cell or cell-pathogen interaction in vivo is an important tool to understand the dynamics of the immune response to infection. Two-photon intravital microscopy (2P-IVM) allows the observation of cell interactions in deep tissue in living animals, while minimizing the photobleaching generated during image acquisition. To date, different models for 2P-IVM of lymphoid and...

Université de Fribourg

Influenza A virus induces autophagosomal targeting of ribosomal proteins

Becker, Andrea C. ; Gannagé, Monique ; Giese, Sebastian ; Hu, Zehan ; Abou-Eid, Shadi ; Roubaty, Carole ; Paul, Petra ; Bühler, Lea ; Gretzmeier, Christine ; Dumit, Veronica I. ; Kaeser-Pebernard, Stéphanie ; Schwemmle, Martin ; Münz, Christian ; Dengjel, Jörn

In: Molecular & Cellular Proteomics, 2018, vol. 17, no. 10, p. 1909–1921

Seasonal epidemics of influenza A virus are a major cause of severe illness and are of high socio-economic relevance. For the design of effective antiviral therapies, a detailed knowledge of pathways perturbed by virus infection is critical. We performed comprehensive expression and organellar proteomics experiments to study the cellular consequences of influenza A virus infection using three...