In: Nanomedicine, 2017, vol. 12, no. 10, p. 1095–1099
As the range of engineered nanoparticles (NPs) designed as specific carriers increases, for example for cell targeting and drug delivery, the question on how many NPs are interacting or are taken up by cells is becoming increasingly important for any potential biomedical application. On one hand, the delivered dose of such NPs to the targeted cells is a key parameter in the assessment of...
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In: ACS Nano, 2017, vol. 11, no. 1, p. 375–383
Nanocarrier design combined with pulmonary drug delivery holds great promise for the treatment of respiratory tract disorders. In particular, targeting of dendritic cells that are key immune cells to enhance or suppress an immune response in the lung is a promising approach for the treatment of allergic diseases. Fluorescently encoded poly(vinyl alcohol) (PVA)-coated gold nanoparticles,...
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In: Journal of Nanobiotechnology, 2017, vol. 15, p. 6
Engineered nanoparticles (NPs) offer site-specific delivery, deposition and cellular uptake due to their unique physicochemical properties and were shown to modulate immune responses. The respiratory tract with its vast surface area is an attractive target organ for innovative immunomodulatory therapeutic applications by pulmonary administration of such NPs, enabling interactions with...
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In: Nanoscale, 2016, vol. 8, no. 36, p. 16416–16426
The impact of nanoparticles (NPs) upon biological systems can be fundamentally associated with their physicochemical parameters. A further often-stated tenet is the importance of NP shape on rates of endocytosis. However, given the convoluted parameters concerning the NP–cell interaction, it is experimentally challenging to attribute any findings to shape alone. Herein we demonstrate that...
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In: Nanoscale, 2016, vol. 8, no. 34, p. 15813–15821
Size polydispersity is a common phenomenon that strongly influences the physicochemical properties of nanoparticles (NPs). We present an analytical approach that is universally applicable to characterizing optically anisotropic round NPs and determines directly the number-averaged size distribution and polydispersity via depolarized dynamic light scattering (DDLS). To demonstrate, we use...
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In: Colloids and Surfaces B: Biointerfaces, 2016, vol. 137, p. 39–49
Nanoparticles possess unique properties beyond that of classical materials, and while these properties can be used for designing a dedicated functionality, they may also pose a problem to living organisms, to human health and the environment. The specific primary routes by which nanoparticles may interact with the human body include inhalation, injection, ingestion and application to the skin....
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In: Particle and Fibre Toxicology, 2015, vol. 12, no. 1, p. 18
The lung epithelial tissue barrier represents the main portal for entry of inhaled nanoparticles (NPs) into the systemic circulation. Thus great efforts are currently being made to determine adverse health effects associated with inhalation of NPs. However, to date very little is known about factors that determine the pulmonary translocation of NPs and their subsequent distribution to secondary...
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In: Chemical Society Reviews, 2015, vol. 44, no. 17, p. 6287–6305
Nanomaterials are finding increasing use for biomedical applications such as imaging, diagnostics, and drug delivery. While it is well understood that nanoparticle (NP) physico-chemical properties can dictate biological responses and interactions, it has been difficult to outline a unifying framework to directly link NP properties to expected in vitro and in vivo outcomes. When introduced to...
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In: Nanomedicine: Nanotechnology, Biology and Medicine, 2015, vol. 11, no. 3, p. 633–644
Engineering nanoparticles (NPs) for immune modulation require a thorough understanding of their interaction(s) with cells. Gold NPs (AuNPs) were coated with polyethylene glycol (PEG), polyvinyl alcohol (PVA) or a mixture of both with either positive or negative surface charge to investigate uptake and cell response in monocyte-derived dendritic cells (MDDCs). Inductively coupled plasma optical...
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In: Particle & Particle Systems Characterization, 2015, vol. 32, no. 3, p. 321–333
When nanomaterials meet the biological world, the cellular interaction of nanoparticles is routinely assessed in in vitro systems. Establishing dose–response relationships requires that the dose of nanoparticles delivered to the cell is accurate and precise. Nanoparticles as such or coated with high molecular-weight compounds are rarely uniform and the influence of heterogeneity, including...
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