Université de Fribourg

The transillumination possibility of imidazole–osmium postfixed tissue and its consequences for the handling of tissue samples

Voigt, Tilman ; Dauber, Wolfgang

In: Microscopy and Microanalysis, 2005, vol. 11(1), p. 42

Osmium postfixation is established as a routine procedure for transmission electron microscopy (TEM). On the one hand, this routine procedure leads to good results for TEM, but on the other hand results in blackened tissue samples that do not allow examination of any structures within the embedded tissue sample by a light microscope. Equivalent fixation results for TEM are achieved with...