Université de Neuchâtel

Chemogenetic protein engineering : optimization of artificial metalloenzymes for oxidation and reduction reactions

Pordea, Anca ; Ward, Thomas R. (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2008 ; Th.2010.

The ability to optimize or to find new catalysts for enantioselective transformations has a major impact in today’s chemistry. Artificial metalloenzymes lie at the interface between organometallic and enzymatic catalysis and have an immense optimization potential, which combines chemical and genetic methods to screen diversity space. In terms of activity, reaction repertoire, substrate range...

Université de Neuchâtel

Evolution rationnelle de métalloenzymes artificielles basées sur la tecnologie Streptavidine-Biotine pour la catalyse énantiosélective par transfert hydrogénant de dérivés carbonylés

Ivanova, Anita ; Ward, Thomas R. (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2007 ; Th.1977.

The production of enantiopure compounds is of increasing importance for applications in the domains of pharmaceutical and food-processing industries, as in material sciences. Production of enantiopure compounds has been boosted with the construction of artificial metalloenzymes. Such catalysts combine the protein environment (bringing some selectivity) and the catalytic activity of metals...

Université de Neuchâtel

Artificial metalloprotein based on biotin-(strept)avidin technology : thermodynamic, kinetic and selectivity considerations

Rusbandi, Untung Edy ; Ward, Thomas R. (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2007 ; Th.1965.

The present work describes our efforts in the design of artificial metalloenzymes based on biotin-(strept)avidin technology. The metal biotinylatedprecursor ensures the activity of the catalyst, whereas the host protein governs the enantioselectivity of artificial metalloenzymes. This study focused on the introduction of chiral spacer between metal precursor and biotin anchor (second generation...

Université de Neuchâtel

Création de métalloenzymes artificielles basées sur la technologie biotine-avidine pour la réduction asymétrique de dérivés carbonylés par transfert hydrogénant

Letondor, Christophe ; Ward, Thomas R. (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2006 ; 1866.

Homogenous- and enzymatic catalysis are in many respects complementary for the synthesis of enantiopure compounds. As the subtle details that govern chiral discrimination are difficult to predict, improving the performance of such catalysts often relies on trial-and-error procedures. Homogenous catalysts are optimized by chemical modification of the first coordination sphere and enzymes can be...