Université de Fribourg

Silica nanoparticles enhance disease resistance in Arabidopsis plants

El-Shetehy, Mohamed ; Moradi, Aboubakr ; Maceroni, Mattia ; Reinhardt, Didier ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara ; Mauch, Felix ; Schwab, Fabienne

In: Nature Nanotechnology, 2020, p. 1–10

In plants, pathogen attack can induce an immune response known as systemic acquired resistance that protects against a broad spectrum of pathogens. In the search for safer agrochemicals, silica nanoparticles (SiO2 NPs; food additive E551) have recently been proposed as a new tool. However, initial results are controversial, and the molecular mechanisms of SiO2 NP-induced disease resistance...

Université de Fribourg

VAPYRIN-like is required for development of the moss Physcomitrella patens

Rathgeb, Ursina ; Chen, Min ; Buron, Flavien ; Feddermann, Nadja ; Schorderet, Martine ; Raisin, Axelle ; Häberli, Gabrielle-Yasymi ; Marc-Martin, Sophie ; Keller, Jean ; Delaux, Pierre-Marc ; Schaefer, Didier G. ; Reinhardt, Didier

In: Development, 2020, vol. 147, no. 11, p. dev184762

The VAPYRIN (VPY) gene in Medicago truncatula and Petunia hybrida is required for arbuscular mycorrhizal (AM) symbiosis. The moss Physcomitrella patens has a close homolog (VPY-like, VPYL), although it does not form AM. Here, we explore the phylogeny of VPY and VPYL in land plants, and study the expression and developmental function of VPYL in P. patens. We show that VPYL is expressed ...

Université de Fribourg

LCO receptors involved in arbuscular mycorrhiza are functional for rhizobia perception in legumes

Girardin, Ariane ; Wang, Tongming ; Ding, Yi ; Keller, Jean ; Buendia, Luis ; Gaston, Mégane ; Ribeyre, Camille ; Gasciolli, Virginie ; Auriac, Marie-Christine ; Vernié, Tatiana ; Bendahmane, Abdelhafid ; Ried, Martina Katharina ; Parniske, Martin ; Morel, Patrice ; Vandenbussche, Michiel ; Schorderet, Martine ; Reinhardt, Didier ; Delaux, Pierre-Marc ; Bono, Jean-Jacques ; Lefebvre, Benoit

In: Current Biology, 2019, vol. 29, no. 24, p. 4249-4259.e5

Bacterial lipo-chitooligosaccharides (LCOs) are key mediators of the nitrogen-fixing root nodule symbiosis (RNS) in legumes. The isolation of LCOs from arbuscular mycorrhizal fungi suggested that LCOs are also signaling molecules in arbuscular mycorrhiza (AM). However, the corresponding plant receptors have remained uncharacterized. Here we show that petunia and tomato mutants in the LysM ...

Université de Fribourg

Strigolactones play an important role in shaping exodermal morphology via a KAI2-dependent pathway

Liu, Guowei ; Stirnemann, Marina ; Gübeli, Christian ; Egloff, Susanne ; Courty, Pierre-Emmanuel ; Aubry, Sylvain ; Vandenbussche, Michiel ; Morel, Patrice ; Reinhardt, Didier ; Martinoia, Enrico ; Borghi, Lorenzo

In: iScience, 2019, vol. 17, p. 144–154

The majority of land plants have two suberized root barriers: the endodermis and the hypodermis (exodermis). Both barriers bear non-suberized passage cells that are thought to regulate water and nutrient exchange between the root and the soil. We learned a lot about endodermal passage cells, whereas our knowledge on hypodermal passage cells (HPCs) is still very scarce. Here we report on...

Université de Fribourg

VAPYRIN Marks an endosomal trafficking compartment involved in arbuscular mycorrhizal symbiosis

Bapaume, Laure ; Laukamm, Sabine ; Darbon, Geoffrey ; Monney, Corinne ; Meyenhofer, Felix ; Feddermann, Nadja ; Chen, Min ; Reinhardt, Didier

In: Frontiers in Plant Science, 2019, vol. 10, p. -

Arbuscular mycorrhiza (AM) is a symbiosis between plants and AM fungi that requires the intracellular accommodation of the fungal partner in the host. For reciprocal nutrient exchange, AM fungi form intracellular arbuscules that are surrounded by the peri-arbuscular membrane. This membrane, together with the fungal plasma membrane, and the space in between, constitute the symbiotic interface,...

Université de Fribourg

Deregulation of MADS-box transcription factor genes in a mutant defective in the WUSCHEL-LIKE HOMEOBOX gene EVERGREEN of Petunia hybrida

Schorderet, Martine ; Muni, Rajasekhara Reddy Duvvuru ; Fiebig, A. ; Reinhardt, Didier

In: Plant Signaling & Behavior, 2018, vol. 13, no. 6, p. e1471299

Angiosperm inflorescences develop in two fundamentally different ways. In monopodial plants, for example in Arabidopsis thaliana, the flowers are initiated as lateral appendages of a central indeterminate inflorescence meristem. In sympodial plants, flowers arise by terminal differentiation of the inflorescence meristem, while further inflorescence development proceeds from new sympodial...

Université de Fribourg

Beneficial services of arbuscular mycorrhizal fungi – from ecology to application

Chen, Min ; Arato, Miguel ; Borghi, Lorenzo ; Nouri, Eva ; Reinhardt, Didier

In: Frontiers in Plant Science, 2018, vol. 9, p. -

Arbuscular mycorrhiza (AM) is the most common symbiotic association of plants with microbes. AM fungi occur in the majority of natural habitats and they provide a range of important ecological services, in particular by improving plant nutrition, stress resistance and tolerance, as well as soil structure and fertility. AM fungi interact with most crop plants including cereals, vegetables, and...

Université de Fribourg

An automated confocal micro-extensometer enables in vivo quantification of mechanical properties with cellular resolution

Robinson, Sarah ; Huflejt, Michal ; Barbier de Reuille, Pierre ; Braybrook, Siobhan A. ; Schorderet, Martine ; Reinhardt, Didier ; Kuhlemeier, Cris

In: The Plant Cell, 2017, vol. 29, no. 12, p. 2959–2973

How complex developmental-genetic networks are translated into organs with specific 3D shapes remains an open question. This question is particularly challenging because the elaboration of specific shapes is in essence a question of mechanics. In plants, this means how the genetic circuitry affects the cell wall. The mechanical properties of the wall and their spatial variation are the key...

Université de Fribourg

Role of the GRAS transcription factor ATA/RAM1 in the transcriptional reprogramming of arbuscular mycorrhiza in Petunia hybrida

Rich, Mélanie K. ; Courty, Pierre-Emmanuel ; Roux, Christophe ; Reinhardt, Didier

In: BMC Genomics, 2017, vol. 18, p. 589

Development of arbuscular mycorrhiza (AM) requires a fundamental reprogramming of root cells for symbiosis. This involves the induction of hundreds of genes in the host. A recently identified GRAS-type transcription factor in Petunia hybrida, ATA/RAM1, is required for the induction of host genes during AM, and for morphogenesis of the fungal endosymbiont. To better understand the role of RAM1...

Université de Fribourg

Diet of arbuscular mycorrhizal fungi: bread and butter ?

Rich, Mélanie K. ; Nouri, Eva ; Courty, Pierre-Emmanuel ; Reinhardt, Didier

In: Trends in Plant Science, 2017, vol. 22, no. 8, p. 652–660

Most plants entertain mutualistic interactions known as arbuscular mycorrhiza (AM) with soil fungi (Glomeromycota) which provide them with mineral nutrients in exchange for reduced carbon from the plant. Mycorrhizal roots represent strong carbon sinks in which hexoses are transferred from the plant host to the fungus. However, most of the carbon in AM fungi is stored in the form of lipids....