Université de Fribourg

GPI anchor attachment in yeast : analysis of Gpi8p, the putative catalytic subunit of the GPI: protein transamidase

Meyer, Urs ; Conzelmann, Andreas (Dir.) ; Puoti, Alessandro (Codir.)

Thèse de doctorat : Université de Fribourg, 2001 ; Nr. 1323.

Membrane anchoring of ceil surface proteins by means of glycosyl phosphatidyl-inositol (GPI) anchors is ubiquitous among eucaryotes. GPI anchoring is essential for yeast viability. In mammals, GPI anchoring is not required at the cellular level, but plays an important role in ceil to ceil and ceil to environment interactions which for example are critical during embryogenesis. GPI proteins can...

Université de Fribourg

Functional analysis of the yeast "Saccharomyces cerevisiae" Gpi8 protein and characterization of the purified GPI-transamidase complex

Fraering, Patrick ; Conzelmann, Andreas (Dir.) ; van der Goot, Françoise Gisou (Codir.) ; Puoti, Alessandro (Codir.)

Thèse de doctorat : Université de Fribourg, 2001 ; no 1354.

Many glycoproteins of lower and higher eucaryotes are attached to the plasma membrane by means of a glycosylphosphatidylinositol (GPI). GPI anchoring of proteins is essential for the growth of Saccharomyces cerevisiae . S.cerevisiae contains about 70 open reading frames predicting GPI proteins and many of these have been found to be cell wall glycoproteins. The precursors of GPI anchored proteins...

Université de Fribourg

Glycosylphosphatidylinositol membrane anchors in "Saccharomyces cerevisiae" : characterization of proteins involved in side chain modifications

Flury, Isabelle ; Conzelmann, Andreas (Dir.) ; Aebi, Markus (Codir.) ; Puoti, Alessandro (Codir.)

Thèse de doctorat : Université de Fribourg, 2001 ; Nr. 1361.

In eukaryotic cells, a subset of proteins is attached to the outer leaflet of the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. Apart from providing stable membrane anchorage, GPI anchors are thought to be associated with other functions, such as signal transduction and protein targeting. The biosynthesis of the GPI anchor and its transfer to proteins is highly conserved in...