Université de Fribourg

Suppressor mutations in Rpf2–Rrs1 or Rpl5 bypass the Cgr1 function for pre-ribosomal 5S RNP-rotation

Thoms, Matthias ; Mitterer, Valentin ; Kater, Lukas ; Falquet, Laurent ; Beckmann, Roland ; Kressler, Dieter ; Hurt, Ed

In: Nature Communications, 2018, vol. 9, no. 1, p. 4094

During eukaryotic 60S biogenesis, the 5S RNP requires a large rotational movement to achieve its mature position. Cryo-EM of the Rix1-Rea1 pre-60S particle has revealed the post-rotation stage, in which a gently undulating α-helix corresponding to Cgr1 becomes wedged between Rsa4 and the relocated 5S RNP, but the purpose of this insertion was unknown. Here, we show that cgr1 deletion in...

Université de Fribourg

A puzzle of life: crafting ribosomal subunits

Kressler, Dieter ; Hurt, Ed ; Baßler, Jochen

In: Trends in Biochemical Sciences, 2017, p. -

The biogenesis of eukaryotic ribosomes is a complicated process during which the transcription, modification, folding, and processing of the rRNA is coupled with the ordered assembly of ∼80 ribosomal proteins (r-proteins). Ribosome synthesis is catalyzed and coordinated by more than 200 biogenesis factors as the preribosomal subunits acquire maturity on their path from the nucleolus to the...

Université de Fribourg

Co-translational capturing of nascent ribosomal proteins by their dedicated chaperones

Pausch, Patrick ; Singh, Ujjwala ; Ahmed, Yasar Luqman ; Pillet, Benjamin ; Murat, Guillaume ; Altegoer, Florian ; Stier, Gunter ; Thoms, Matthias ; Hurt, Ed ; Sinning, Irmgard ; Bange, Gert ; Kressler, Dieter

In: Nature Communications, 2015, vol. 6, p. 7494

Exponentially growing yeast cells produce every minute >160,000 ribosomal proteins. Owing to their difficult physicochemical properties, the synthesis of assembly-competent ribosomal proteins represents a major challenge. Recent evidence highlights that dedicated chaperone proteins recognize the N-terminal regions of ribosomal proteins and promote their soluble expression and delivery to the...

Université de Fribourg

Symportin 1 chaperones 5S RNP assembly during ribosome biogenesis by occupying an essential rRNA-binding site

Calviño, Fabiola R. ; Kharde, Satyavati ; Ori, Alessandro ; Hendricks, Astrid ; Wild, Klemens ; Kressler, Dieter ; Bange, Gert ; Hurt, Ed ; Beck, Martin ; Sinning, Irmgard

In: Nature Communications, 2015, vol. 6, p. -

During 60S biogenesis, mature 5S RNP consisting of 5S RNA, RpL5 and RpL11, assembles into a pre-60S particle, where docking relies on RpL11 interacting with helix 84 (H84) of the 25S RNA. How 5S RNP is assembled for recruitment into the pre-60S is not known. Here we report the crystal structure of a ternary symportin Syo1–RpL5-N–RpL11 complex and provide biochemical and structural insights...

Université de Fribourg

Synchronizing nuclear import of ribosomal proteins with ribosome assembly

Kressler, Dieter ; Bange, Gert ; Ogawa, Yutaka ; Stjepanovic, Goran ; Bradatsch, Bettina ; Pratte, Dagmar ; Amlacher, Stefan ; Strauß, Daniela ; Yoneda, Yoshihiro ; Katahira, Jun ; Sinning, Irmgard ; Hurt, Ed

In: Science, 2012, vol. 338, no. 6107, p. 666-671

Ribosomal proteins are synthesized in the cytoplasm, before nuclear import and assembly with ribosomal RNA (rRNA). Little is known about coordination of nucleocytoplasmic transport with ribosome assembly. Here, we identify a transport adaptor, symportin 1 (Syo1), that facilitates synchronized coimport of the two 5S-rRNA binding proteins Rpl5 and Rpl11. In vitro studies revealed that Syo1...

Université de Fribourg

Structural basis for the molecular evolution of SRP-GTPase activation by protein

Bange, Gert ; Kümmerer, Nico ; Grudnik, Przemyslaw ; Lindner, Robert ; Petzold, Georg ; Kressler, Dieter ; Hurt, Ed ; Wild, Klemens ; Sinning, Irmgard

In: Nature Structural & Molecular Biology, 2011, vol. 18, p. 1376–1380

Small G proteins have key roles in signal transduction pathways. They are switched from the signaling 'on' to the non-signaling 'off' state when GTPase-activating proteins (GAPs) provide a catalytic residue. The ancient signal recognition particle (SRP)-type GTPases form GTP-dependent homo- and heterodimers and deviate from the canonical switch paradigm in that no GAPs have been identified. Here...

Université de Fribourg

The power of AAA-ATPases on the road of pre-60S ribosome maturation — Molecular machines that strip pre-ribosomal particles

Kressler, Dieter ; Hurt, Ed ; Bergler, Helmut ; Baßler, Jochen

In: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2011, p. -

The biogenesis of ribosomes is a fundamental cellular process, which provides the molecular machines that synthesize all cellular proteins. The assembly of eukaryotic ribosomes is a highly complex multi-step process that requires more than 200 ribosome biogenesis factors, which mediate a broad spectrum of maturation reactions. The participation of many energy-consuming enzymes (e.g. AAA-type...

Université de Fribourg

Driving ribosome assembly

Kressler, Dieter ; Hurt, Ed ; Baβler, Jochen

In: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2010, p. -

Ribosome biogenesis is a fundamental process that provides cells with the molecular factories for cellular protein production. Accordingly, its misregulation lies at the heart of several hereditary diseases (e.g., Diamond-Blackfan anemia). The process of ribosome assembly comprises the processing and folding of the pre-rRNA and its concomitant assembly with the ribosomal proteins. Eukaryotic...