Université de Fribourg

Tissue-specific transcription footprinting using RNA PoI DamID (RAPID) in Caenorhabditis elegans

Gómez-Saldivar, Georgina ; Osuna-Luque, Jaime ; Semple, Jennifer I. ; Glauser, Dominique A. ; Jarriault, Sophie ; Meister, Peter

In: Genetics, 2020, vol. 216, no. 4, p. 931–945

Differential gene expression across cell types underlies development and cell physiology in multicellular organisms. Caenorhabditis elegans is a powerful, extensively used model to address these biological questions. A remaining bottleneck relates to the difficulty to obtain comprehensive tissue-specific gene transcription data, since available methods are still challenging to execute and/or...

Université de Fribourg

Identification of avoidance genes through neural pathway-specific forward optogenetics

Marques, Filipe ; Saro, Gabriella ; Lia, Andrei-Stefan ; Poole, Richard J. ; Falquet, Laurent ; Glauser, Dominique A.

In: PLOS Genetics, 2019, vol. 15, no. 12, p. e1008509

Understanding how the nervous system bridges sensation and behavior requires the elucidation of complex neural and molecular networks. Forward genetic approaches, such as screens conducted in C. elegans, have successfully identified genes required to process natural sensory stimuli. However, functional redundancy within the underlying neural circuits, which are often organized with multiple...

Université de Fribourg

Tissue-specific isoforms of the single C. elegans Ryanodine receptor gene unc-68 control specific functions

Marques, Filipe ; Thapliyal, Saurabh ; Javer, Avelino ; Shrestha, Priyanka ; Brown, André E. X. ; Glauser, Dominique A.

In: PLOS Genetics, 2020, vol. 16, no. 10, p. e1009102

Ryanodine receptors (RyR) are essential regulators of cellular calcium homeostasis and signaling. Vertebrate genomes contain multiple RyR gene isoforms, expressed in different tissues and executing different functions. In contrast, invertebrate genomes contain a single RyR-encoding gene and it has long been proposed that different transcripts generated by alternative splicing may diversify...

Université de Fribourg

Specific ion channels control sensory gain, sensitivity, and kinetics in a tonic thermonociceptor

Saro, Gabriella ; Lia, Andrei-Stefan ; Thapliyal, Saurabh ; Marques, Filipe ; Busch, Karl Emanuel ; Glauser, Dominique A.

In: Cell Reports, 2020, vol. 30, no. 2, p. 397-408.e4

Pain sensation and aversive behaviors entail the activation of nociceptor neurons, whose function is largely conserved across animals. The functional heterogeneity of nociceptors and ethical concerns are challenges for their study in mammalian models. Here, we investigate the function of a single type of genetically identified C. elegans thermonociceptor named FLP. Using calcium imaging in...

Université de Fribourg

Dual color neural activation and behavior control with chrimson and CoChR in Caenorhabditis elegans

Schild, Lisa C. ; Glauser, Dominique A.

In: Genetics, 2015, vol. 200, no. 4, p. 1029–1034

By enabling a tight control of cell excitation, optogenetics is a powerful approach to study the function of neurons and neural circuits. With its transparent body, a fully mapped nervous system, easily quantifiable behaviors and many available genetic tools, Caenorhabditis elegans is an extremely well-suited model to decipher the functioning logic of the nervous system with optogenetics. Our...

Université de Fribourg

A conserved role for p48 homologs in protecting dopaminergic neurons from oxidative stress

Dib, Peter Bou ; Gnägi, Bettina ; Daly, Fiona ; Sabado, Virginie ; Tas, Damla ; Glauser, Dominique A. ; Nagoshi, Peter Meister, Emi

In: PLoS Genet, 2014, vol. 10, no. 10, p. e1004718

Parkinson's disease (PD) is the most common neurodegenerative movement disorder characterized by the progressive loss of dopaminergic (DA) neurons. Both environmental and genetic factors are thought to contribute to the pathogenesis of PD. Although several genes linked to rare familial PD have been identified, endogenous risk factors for sporadic PD, which account for the majority of PD cases,...

Université de Fribourg

The multiplicity of alternative splicing decisions in Caenorhabditis elegans is linked to specific intronic regulatory motifs and minisatellites

Glauser, Dominique A.

In: BMC Genomics, 2014, vol. 15, no. 1, p. 364

Background: alternative splicing diversifies the pool of messenger RNA molecules encoded by individual genes. This diversity is particularly high when multiple splicing decisions cause a combinatorial arrangement of several alternate exons. We know very little on how the multiple decisions occurring during the maturation of single transcripts are coordinated and whether specific sequence elements...

Université de Fribourg

Dynamic switching between escape and avoidance regimes reduces Caenorhabditis elegans exposure to noxious heat

Schild, Lisa C. ; Glauser, Dominique A.

In: Nature Communications, 2013, vol. 4, p. -

To survive, animals need to minimize exposure to damaging agents. They can either stay away from noxious stimuli (defined as avoidance), requiring the detection of remote warning cues, or run away upon exposure to noxious stimuli (defined as escape). Here we combine behavioural quantitative analyses, simulations and genetics to determine how Caenorhabditis elegans minimizes exposure to noxious...

Université de Fribourg

Acr-23 encodes a Monepantel-sensitive channel in Caenorhabditis elegans

Rufener, Lucien ; Bedoni, Nicola ; Baur, Roland ; Rey, Samantha ; Glauser, Dominique A. ; Bouvier, Jacques ; Beech, Robin ; Sigel, Erwin ; Puoti, Alessandro

In: PLoS Pathog, 2013, vol. 9, no. 8, p. e1003524

Monepantel is a member of the recently identified class of anthelmintics known as the amino-acetonitrile derivatives (AADs). Monepantel controls all major gastro-intestinal nematodes in sheep including those that are resistant to the classical anthelmintics. Previous studies have shown that the Caenorhabditis elegans acr-23 and the Haemonchus contortus Hco-mptl-1 genes may be prominent targets of...

Université de Fribourg

Intragenic alternative splicing coordination is essential for Caenorhabditis elegans slo-1 gene function

Glauser, Dominique A. ; Johnson, Brandon E. ; Aldrich, Richard W. ; Goodman, Miriam B.

In: Proceedings of the National Academy of Sciences, 2011, vol. 108, no. 51, p. 20790-20795

Alternative splicing is critical for diversifying eukaryotic proteomes, but the rules governing and coordinating splicing events among multiple alternate splice sites within individual genes are not well understood. We developed a quantitative PCR-based strategy to quantify the expression of the 12 transcripts encoded by the Caenorhabditis elegans slo-1 gene, containing three alternate splice...