Université de Fribourg

GPI anchor attachment in yeast : analysis of Gpi8p, the putative catalytic subunit of the GPI: protein transamidase

Meyer, Urs ; Conzelmann, Andreas (Dir.) ; Puoti, Alessandro (Codir.)

Thèse de doctorat : Université de Fribourg, 2001 ; Nr. 1323.

Membrane anchoring of ceil surface proteins by means of glycosyl phosphatidyl-inositol (GPI) anchors is ubiquitous among eucaryotes. GPI anchoring is essential for yeast viability. In mammals, GPI anchoring is not required at the cellular level, but plays an important role in ceil to ceil and ceil to environment interactions which for example are critical during embryogenesis. GPI proteins can...

Université de Fribourg

The tip60 HAT-complex is recruited to chromatin by the transcription factors E2F and Myc

Taubert, Stefan ; Conzelmann, Andreas (Dir.)

Thèse de doctorat : Université de Fribourg, 2003 ; no 1409.

The oncogenic transcription factors E2F and Myc bind to specific DNA sequences at distinct times during the G1 phase of the mammalian cell cycle, and regulate transcription of a plethora of target genes. E2F and Myc associate with TRRAP, a subunit of distinct macromolecular complexes that contain the histone acetyltransferases (HATs) Gcn5/PCAF or Tip60. During the course of my thesis I...

Université de Fribourg

Functional analysis of the yeast "Saccharomyces cerevisiae" Gpi8 protein and characterization of the purified GPI-transamidase complex

Fraering, Patrick ; Conzelmann, Andreas (Dir.) ; van der Goot, Françoise Gisou (Codir.) ; Puoti, Alessandro (Codir.)

Thèse de doctorat : Université de Fribourg, 2001 ; no 1354.

Many glycoproteins of lower and higher eucaryotes are attached to the plasma membrane by means of a glycosylphosphatidylinositol (GPI). GPI anchoring of proteins is essential for the growth of Saccharomyces cerevisiae . S.cerevisiae contains about 70 open reading frames predicting GPI proteins and many of these have been found to be cell wall glycoproteins. The precursors of GPI anchored proteins...

Université de Fribourg

Glycosylphosphatidylinositol membrane anchors in "Saccharomyces cerevisiae" : characterization of proteins involved in side chain modifications

Flury, Isabelle ; Conzelmann, Andreas (Dir.) ; Aebi, Markus (Codir.) ; Puoti, Alessandro (Codir.)

Thèse de doctorat : Université de Fribourg, 2001 ; Nr. 1361.

In eukaryotic cells, a subset of proteins is attached to the outer leaflet of the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. Apart from providing stable membrane anchorage, GPI anchors are thought to be associated with other functions, such as signal transduction and protein targeting. The biosynthesis of the GPI anchor and its transfer to proteins is highly conserved in...

Université de Fribourg

The dynamic localization and novel functions of the Ipl1/Aurora protein kinase

Buvelot, Stéphanie ; Conzelmann, Andreas (Dir.)

Thèse de doctorat : Université de Fribourg : 2003 ; no 1400.

Proper chromosome segregation is a crucial event for accurate cell division. Defects in chromosome segregation lead to genetic instability and aneuploidy and are correlated with cancer. The laboratory studies chromosome segregation in the budding yeast Saccharomyces cerevisiae. Since this process is highly conserved among eukaryotes, studies in yeast will provide fundamental understanding of this...