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Université de Fribourg

Cell-Permeant and photocleavable chemical inducer of dimerization

Zimmermann, Mirjam ; Cal, Ruben ; Janett, Elia ; Hoffmann, Viktor ; Bochet, Christian G. ; Constable, Edwin ; Beaufils, Florent ; Wymann, Matthias P.

In: Angewandte Chemie International Edition, 2014, vol. 53, no. 18, p. 4717–4720

Chemical inducers of dimerization (CIDs) have been developed to orchestrate protein dimerization and translocation. Here we present a novel photocleavable HaloTag- and SNAP-tag-reactive CID (MeNV-HaXS) with excellent selectivity and intracellular reactivity. Excitation at 360 nm cleaves the methyl-6-nitroveratryl core of MeNV-HaXS. MeNV-HaXS covalently links HaloTag- and SNAP-tag fusion...

Université de Fribourg

Disease resistance of Arabidopsis to Phytophthora brassicae is established by the sequential action of indole glucosinolates and camalexin

Schlaeppi, Klaus ; Abou-Mansour, Eliane ; Buchala, Antony J. ; Mauch, Felix

In: The Plant Journal, 2010, vol. 62, no. 5, p. 840 - 851

We have analysed the role of tryptophan-derived secondary metabolites in disease resistance of Arabidopsis to the oomycete pathogen Phytophthora brassicae. Transcript analysis revealed that genes encoding enzymes involved in tryptophan, camalexin and indole glucosinolate (iGS) biosynthesis are coordinately induced in response to P. brassicae. However, a deficiency in either camalexin or iGS...

Université de Fribourg

Bax function in the absence of mitochondria in the primitive protozoan Giardia lamblia

Hehl, Adrian B. ; Regos, Attila ; Schraner, Elisabeth ; Schneider, André

In: PLoS ONE, 2007, vol. 2, no. 5, p. e488

Bax-induced permeabilization of the mitochondrial outer membrane and release of cytochrome c are key events in apoptosis. Although Bax can compromise mitochondria in primitive unicellular organisms that lack a classical apoptotic machinery, it is still unclear if Bax alone is sufficient for this, or whether additional mitochondrial components are required. The protozoan parasite Giardia lamblia...

Université de Fribourg

Bcl-2 family members: intracellular targeting, membrane-insertion, and changes in subcellular localization

Schinzel, Anna ; Kaufmann, Thomas ; Borner, Christoph

In: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2004, vol. 1644, no. 2, p. 95-105

The members of the Bcl-2 family of proteins are crucial regulators of apoptosis. In order to determine cell fate, these proteins must be targeted to distinct intracellular membranes, including the mitochondrial outer membrane (MOM), the membrane of the endoplasmic reticulum (ER) and its associated nuclear envelope. The targeting sequences and mechanisms that mediate the specificity of these...