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Université de Fribourg

High colonization rate and heterogeneity of ESBL- and carbapenemase-producing enterobacteriaceae isolated from gull feces in Lisbon, Portugal

Aires-de-Sousa, Marta ; Fournier, Claudine ; Lopes, Elizeth ; Lencastre, Hermínia de ; Nordmann, Patrice ; Poirel, Laurent

In: Microorganisms, 2020, vol. 8, no. 10, p. 1487

In order to evaluate whether seagulls living on the Lisbon coastline, Portugal, might be colonized and consequently represent potential spreaders of multidrug-resistant bacteria, a total of 88 gull fecal samples were screened for detection of extended- spectrum β-lactamase (ESBL)- or carbapenemase-producing Enterobacteriaceae for methicillin-resistant Staphylococcus aureus (MRSA) and for...

Université de Fribourg

Epidemiology of extended-spectrum β-lactamase-producing Enterobacteriaceae among healthcare students, at the Portuguese Red Cross Health School of Lisbon, Portugal

Fournier, Claudine ; Aires de Sousa, Marta ; Fuster Escriva, Begoña ; Sales, Leila ; Nordmanna, Patrice ; Poirel, Laurent

In: Journal of Global Antimicrobial Resistance, 2020, vol. 22, p. 733–737

Objective: The aim of the present study was to prospectively evaluate the prevalence of intestinal carriage by extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae among Portuguese students attending a Bachelors’ course in healthcare, and to determine the molecular features of ESBL-producing isolates.Methods: One-hundred and eleven faecal samples recovered from Portuguese...

Université de Fribourg

Epidemiology of carbapenemase-producing Klebsiella pneumoniae in northern Portugal: Predominance of KPC-2 and OXA-48

Lopes, Elizeth ; Saavedra, Maria José ; Costa, Eliana ; Lencastre, Hermíniade ; Poirel, Laurent ; Aires-de-Sousa, Marta

In: Journal of Global Antimicrobial Resistance, 2020, vol. 22, p. 349–353

Objectives: To provide, for the first time, data on the molecular epidemiology of carbapenemase-producing Klebsiella pneumoniae clinical isolates from the northern region of Portugal (Trás-os-Montes and Alto Douro).Methods: A total of 106 carbapenemase-producing K. pneumoniae isolates recovered from clinical samples and rectal swabs between January 2018 and March 2019 were included in this...

Université de Fribourg

Occurrence of NDM-1-producing Morganella morganii and Proteus mirabilis in a single patient in Portugal: probable in vivo transfer by conjugation

Aires-de-Sousa, Marta ; Ortiz de la Rosa, José Manuel ; Goncalves, Maria Luísa ; Costa, Augusto ; Nordmann, Patrice ; Poirel, Laurent

In: Journal of Antimicrobial Chemotherapy, 2020, vol. 75, no. 4, p. 903–906

To decipher the genetics of acquisition of carbapenemase-encoding genes identified in two carbapenem-resistant Enterobacteriaceae recovered from a single patient in Portugal.Methods: Carbapenemase genes were searched by PCR assays and mating-out assays were performed to further characterize the plasmid support of the carbapenemase genes. Genetic characterization of the plasmid supports was...

Université de Fribourg

Occurrence of CTX-M-15- and MCR-1-producing Enterobacterales in pigs in Portugal: evidence of direct links with antibiotic selective pressure

Fournier, Claudine ; Aires-de-Sousa, Marta ; Nordmann, Patrice ; Poirel, Laurent

In: International Journal of Antimicrobial Agents, 2020, vol. 55, no. 2, p. 105802

AimsTo undertake a prospective analysis of the occurrence of colistin-resistant and extended-spectrum ß-lactamase (ESBL)-producing Enterobacterales colonizing pigs at two farms in Portugal, and to evaluate the putative correlations with usage of different antibiotics.Materials and methods: One hundred and two faecal samples recovered from two different Portuguese pig farms were screened for...

Université de Fribourg

Epidemiology of Carbapenemase-Producing Klebsiella pneumoniae in a Hospital, Portugal

Aires-de-Sousa, Marta ; Ortiz de la Rosa, José Manuel ; Gonçalves, Maria Luísa ; Pereira, Ana Luísa ; Nordmann, Patrice ; Poirel, Laurent

In: Emerging Infectious Diseases, 2019, vol. 25, no. 9, p. 1632–1638

We aimed to provide updated epidemiologic data on carbapenem-resistant Klebsiella pneumoniae in Portugal by characterizing all isolates (N = 46) recovered during 2013– 2018 in a 123-bed hospital in Lisbon. We identified blaKPC-3 (n = 36), blaOXA-181 (n = 9), and blaGES-5 (n = 8) carbapenemase genes and observed co-occurrence of blaKPC-3 and blaGES-5 in 7 isolates. A single GES-5–producing...