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Université de Fribourg

A standard numbering scheme for class c β-lactamases

Mack, Andrew R. ; Barnes, Melissa D. ; Taracila, Magdalena A. ; Hujer, Andrea M. ; Hujer, Kristine M. ; Cabot, Gabriel ; Feldgarden, Michael ; Haft, Daniel H. ; Klimke, William ; Akker, Focco van den ; Vila, Alejandro J. ; Smania, Andrea ; Haider, Shozeb ; Papp-Wallace, Krisztina M. ; Bradford, Patricia A. ; Rossolini, Gian Maria ; Docquier, Jean-Denis ; Frère, Jean-Marie ; Galleni, Moreno ; Hanson, Nancy D. ; Oliver, Antonio ; Plésiatyz, Patrick ; Poirel, Laurent ; Nordmann, Patrice ; Palzkill, Timothy G. ; Jacoby, George A. ; Bush, Karen ; Bonomo, Robert A.

In: Antimicrobial Agents and Chemotherapy, 2020, vol. 64, no. 3, p. -

Unlike for classes A and B, a standardized amino acid numbering scheme has not been proposed for the class C (AmpC) β-lactamases, which complicates communication in the field. Here, we propose a scheme developed through a collaborative approach that considers both sequence and structure, preserves traditional numbering of catalytically important residues (Ser64, Lys67, Tyr150, and Lys315),...

Université de Fribourg

Enigmatic dinosaur precursors bridge the gap to the origin of Pterosauria

Ezcurra, Martín D. ; Nesbitt, Sterling J. ; Bronzati, Mario ; Vecchia, Fabio Marco Dalla ; Agnolin, Federico L. ; Benson, Roger B. J. ; Egli, Federico Brissón ; Cabreira, Sergio F. ; Evers, Serjoscha W. ; Gentil, Adriel R. ; Irmis, Randall B. ; Martinelli, Agustín G. ; Novas, Fernando E. ; Silva, Lúcio Roberto da ; Smith, Nathan D. ; Stocker, Michelle R. ; Turner, Alan H. ; Langer, Max C.

In: Nature, 2020, vol. 588, no. 2020-7838, p. 445–449

Pterosaurs were the first vertebrates to evolve powered flight1 and comprised one of the main evolutionary radiations in terrestrial ecosystems of the Mesozoic era (approximately 252–66 million years ago), but their origin has remained an unresolved enigma in palaeontology since the nineteenth century2,3,4. These flying reptiles have been hypothesized to be the close relatives of a wide...

Université de Fribourg

Directing single-molecule emission with dna origami-assembled optical antennas

Hübner, Kristina ; Pilo-Pais, Mauricio ; Selbach, Florian ; Liedl, Tim ; Tinnefeld, Philip ; Stefani, Fernando D. ; Acuna, Guillermo P.

In: Nano Letters, 2019, vol. 19, no. 9, p. 6629–6634

We demonstrate the capability of DNA self-assembled optical antennas to direct the emission of an individual fluorophore, which is free to rotate. DNA origami is used to fabricate optical antennas composed of two colloidal gold nanoparticles separated by a predefined gap and to place a single Cy5 fluorophore near the gap center. Although the fluorophore is able to rotate, its excitation and...

Consortium of Swiss Academic Libraries

Experimental tissue engineering of fetal skin

Mazzone, L. ; Pontiggia, L. ; Reichmann, E. ; Ochsenbein-Kölble, N. ; Moehrlen, U. ; Meuli, M.

In: Pediatric Surgery International, 2014, vol. 30, no. 12, p. 1241-1247