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Université de Fribourg

Contribution of hydrogen cyanide to the antagonistic activity of Pseudomonas strains against Phytophthora infestans

Anand, Abhishek ; Chinchilla, Delphine ; Tan, Christopher ; Mène-Saffrané, Laurent ; L’Haridon, Floriane ; Weisskopf, Laure

In: Microorganisms, 2020, vol. 8, no. 8, p. 1144

Plants face many biotic and abiotic challenges in nature; one of them is attack by disease-causing microbes. Phytophthora infestans, the causal agent of late blight is one of the most prominent pathogens of the potato responsible for multi-billion-dollar losses every year. We have previously reported that potato-associated Pseudomonas strains inhibited P. infestans at various developmental...

Université de Fribourg

Linking comparative genomics of nine potato-associated pseudomonas isolates with their differing biocontrol potential against late blight

Vrieze, Mout De ; Varadarajan, Adithi R. ; Schneeberger, Kerstin ; Bailly, Aurélien ; Rohr, Rudolf P. ; Ahrens, Christian H. ; Weisskopf, Laure

In: Frontiers in Microbiology, 2020, vol. 11, p. 857

For plants, the advantages of associating with beneficial bacteria include plant growth promotion, reduction of abiotic and biotic stresses and enhanced protection against various pests and diseases. Beneficial bacteria rightly equipped for successful plant colonization and showing antagonistic activity toward plant pathogens seem to be actively recruited by plants. To gain more insights into...

Université de Fribourg

Pfm-like enzymes are a novel family of subclass b2 metallo-β-lactamases from Pseudomonas synxantha belonging to the Pseudomonas fluorescens complex

Poirel, Laurent ; Palmieri, Mattia ; Brilhante, Michael ; Masseron, Amandine ; Perreten, Vincent ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2020, vol. 64, no. 2, p. -

A carbapenem-resistant Pseudomonas synxantha isolate recovered from chicken meat produced the novel carbapenemase PFM-1. That subclass B2 metallo-β- lactamase shared 71% amino acid identity with β-lactamase Sfh-1 from Serratia fonticola. The blaPFM-1 gene was chromosomally located and likely acquired. Variants of PFM-1 sharing 90% to 92% amino acid identity were identified in bacterial ...

Université de Fribourg

Biocontrol activity of three Pseudomonas in a newly assembled collection of Phytophthora infestans isolates

De Vrieze, Mout ; Gloor, Ramona ; Massana Codina, Josep ; Torriani, Stefano ; Gindro, Katia ; L?Haridon, Floriane ; Bailly, Aurélien ; Weisskopf, Laure

In: Phytopathology, 2019, vol. 109, no. 9, p. 1555–1565

Late blight caused by the oomycete Phytophthora infestans constitutes the greatest threat to potato production worldwide. Considering the increasing concerns regarding the emergence of novel fungicide-resistant genotypes and the general demand for reducing inputs of synthetic and copper-based fungicides, the need for alternative control methods is acute. Several bacterial antagonists have...

Università della Svizzera italiana

Opening study on the development of a new biosensor for metal toxicity based on pseudomonas fluorescens pyoverdine

Chiadò, Alessandro ; Varani, Luca ; Bosco, Francesco ; Marmo, Luca

In: Biosensors, 2013, vol. 3, no. 4, p. 385-399

To date, different kinds of biosensing elements have been used effectively for environmental monitoring. Microbial cells seem to be well-suited for this task: they are cheap, adaptable to variable field conditions and give a measurable response to a broad number of chemicals. Among different pollutants, heavy metals are still a major problem for the environment. A reasonable starting point...

Université de Fribourg

Removal of a subset of non-essential genes fully attenuates a highly virulent mycoplasma strain

Jores, Joerg ; Ma, Li ; Ssajjakambwe, Paul ; Schieck, Elise ; Liljander, Anne ; Chandran, Suchismita ; Stoffel, Michael H. ; Cippa, Valentina ; Arfi, Yonathan ; Assad-Garcia, Nacyra ; Falquet, Laurent ; Sirand-Pugnet, Pascal ; Blanchard, Alain ; Lartigue, Carole ; Posthaus, Horst ; Labroussaa, Fabien ; Vashee, Sanjay

In: Frontiers in Microbiology, 2019, vol. 10, p. -

Mycoplasmas are the smallest free-living organisms and cause a number of economically important diseases affecting humans, animals, insects, and plants. Here, we demonstrate that highly virulent Mycoplasma mycoides subspecies capri (Mmc) can be fully attenuated via targeted deletion of non-essential genes encoding, among others, potential virulence traits. Five genomic regions, representing...

Université de Fribourg

Combining different potato-associated pseudomonas strains for improved biocontrol of Phytophthora infestans

Vrieze, Mout De ; Germanier, Fanny ; Vuille, Nicolas ; Weisskopf, Laure

In: Frontiers in Microbiology, 2018, vol. 9, p. -

Late blight caused by Phytophthora infestans is considered as the most devastating disease of potato and is a re-emerging problem worldwide. Current late blight control practices rely mostly on synthetic fungicides or copper-based products, but growing awareness of the negative impact of these compounds on the environment has led to the search for alternative control measures. A collection of...