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Polarized Actin Structural Dynamics in Response to Cyclic Uniaxial Stretch

Huang, Lawrence ; Helmke, Brian

In: Cellular and Molecular Bioengineering, 2015, vol. 8, no. 1, p. 160-177

Université de Fribourg

Particle surfaces to study macrophage adherence, migration, and clearance

Septiadi, Dedy ; Lee, Aaron ; Spuch‐Calvar, Miguel ; Moore, Thomas Lee ; Spiaggia, Giovanni ; Abdussalam, Wildan ; Rodriguez‐Lorenzo, Laura ; Taladriz‐Blanco, Patricia ; Rothen‐Rutishauser, Barbara ; Petri‐Fink, Alke

In: Advanced Functional Materials, 2020, p. 2002630

Nanoparticle adsorption to substrates pose a unique challenge to understand uptake mechanisms as it involves the organization of complex cytoskeletal components by cells to perform endocytosis/phagocytosis. In particular, it is not well‐understood from a cell mechanics perspective how the adhesion of particles on substrate will influence the ease of material clearance. By using a particle...

Université de Fribourg

The yeast cell wall protein Pry3 inhibits mating through highly conserved residues within the CAP domain

Cottier, Stéphanie ; Darwiche, Rabih ; Meyenhofer, Felix ; Debelyy, Mykhaylo O. ; Schneiter, Roger

In: Biology Open, 2020, vol. 9, no. 6, p. bio053470

Members of the CAP/SCP/TAPS superfamily have been implicated in many different physiological processes, including pathogen defense, sperm maturation and fertilization. The mode of action of this class of proteins, however, remains poorly understood. The genome of Saccharomyces cerevisiae encodes three CAP superfamily members, Pry1-3. We have previously shown that Pry1 function is required...

Université de Fribourg

The transcription factor Spt4-Spt5 complex regulates the expression of ATG8 and ATG41

Wen, Xin ; Gatica, Damián ; Yin, Zhangyuan ; Hu, Zehan ; Dengjel, Jörn ; Klionsky, Daniel J.

In: Autophagy, 2019, vol. 0, no. 0, p. 1–14

Macroautophagy/autophagy, a highly conserved dynamic process, is one of the major degradative pathways in cells. So far, over 40 autophagy-related (ATG) genes have been identified in Saccharomyces cerevisiae, most of which have homologs in more complex eukaryotes. Autophagy plays a crucial role in cell survival and maintenance, and its dysfunction is related to various diseases, indicating...

Università della Svizzera italiana

MiR-221 influences effector functions and actin cytoskeleton in mast cells

Mayoral, Ramon J. ; Deho, Lorenzo ; Rusca, Nicole ; Bartonicek, Nenad ; Saini, Harpreet Kaur ; Enright, Anton J. ; Monticelli, Silvia

In: Plos one, 2011, vol. 6, no. 10, p. e26133

Mast cells have essential effector and immunoregulatory functions in IgE-associated allergic disorders and certain innate and adaptive immune responses, but the role of miRNAs in regulating mast cell functions is almost completely unexplored. To examine the role of the activation-induced miRNA miR-221 in mouse mast cells, we developed robust lentiviral systems for miRNA overexpression and...

Université de Fribourg

Regulation of global CD8+ T-cell positioning by the actomyosin cytoskeleton

Stein, Jens V. ; Ruef, Nora

In: Immunological Reviews, 2019, vol. 289, no. 1, p. 232–249

CD8+ T cells have evolved as one of the most motile mammalian cell types, designed to continuously scan peptide–major histocompatibility complexes class I on the surfaces of other cells. Chemoattractants and adhesion molecules direct CD8+ T‐cell homing to and migration within secondary lymphoid organs, where these cells colocalize with antigen‐presenting dendritic cells in confined...

Université de Fribourg

Arp3 controls the podocyte architecture at the kidney filtration barrier

Schell, Christoph ; Sabass, Benedikt ; Helmstaedter, Martin ; Geist, Felix ; Abed, Ahmed ; Yasuda-Yamahara, Mako ; Sigle, August ; Maier, Jasmin I. ; Grahammer, Florian ; Siegerist, Florian ; Artelt, Nadine ; Endlich, Nicole ; Kerjaschki, Dontscho ; Arnold, Hans-Henning ; Dengjel, Jörn ; Rogg, Manuel ; Huber, Tobias B.

In: Developmental Cell, 2018, vol. 47, no. 6, p. 741-757.e8

Podocytes, highly specialized epithelial cells, build the outer part of the kidney filtration barrier and withstand high mechanical forces through a complex network of cellular protrusions. Here, we show that Arp2/3-dependent actin polymerization controls actomyosin contractility and focal adhesion maturation of podocyte protrusions and thereby regulates formation, maintenance, and capacity...