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Université de Fribourg

Conserved functions of ether lipids and sphingolipids in the early secretory pathway

Jiménez-Rojo, Noemi ; Leonetti, Manuel D. ; Zoni, Valeria ; Colom, Adai ; Feng, Suihan ; Iyengar, Namrata R. ; Matile, Stefan ; Roux, Aurélien ; Vanni, Stefano ; Weissman, Jonathan S. ; Riezman, Howard

In: Current Biology, 2020, vol. 30, no. 19, p. 3775-3787.e7

Sphingolipids play important roles in physiology and cell biology, but a systematic examination of their functions is lacking. We performed a genome-wide CRISPRi screen in sphingolipid-depleted human cells and identified hypersensitive mutants in genes of membrane trafficking and lipid biosynthesis, including ether lipid synthesis. Systematic lipidomic analysis showed a coordinate regulation...

Université de Fribourg

Raft-like lipid microdomains drive autophagy initiation via AMBRA1-ERLIN1 molecular association within MAMs

Manganelli, Valeria ; Matarrese, Paola ; Antonioli, Manuela ; Gambardella, Lucrezia ; Vescovo, Tiziana ; Gretzmeier, Christine ; Longo, Agostina ; Capozzi, Antonella ; Recalchi, Serena ; Riitano, Gloria ; Misasi, Roberta ; Dengjel, Jörn ; Malorni, Walter ; Fimia, Gian Maria ; Sorice, Maurizio ; Garofalo, Tina

In: Autophagy, 2020, p. 1-21

Mitochondria-associated membranes (MAMs) are essential communication subdomains of the endoplasmic reticulum (ER) that interact with mitochondria. We previously demonstrated that, upon macroautophagy/autophagy induction, AMBRA1 is recruited to the BECN1 complex and relocalizes to MAMs, where it regulates autophagy by interacting with raft-like components. ERLIN1 is an endoplasmic reticulum...

Université de Fribourg

S-methyl methanethiosulfonate: promising late blight inhibitor or broad range toxin?

Joller, Charlotte ; Vrieze, Mout De ; Moradi, Aboubakr ; Fournier, Claudine ; Chinchilla, Delphine ; L’Haridon, Floriane ; Bruisson, Sebastien ; Weisskopf, Laure

In: Pathogens, 2020, vol. 9, no. 6, p. 496

(1) Background: S-methyl methanethiosulfonate (MMTS), a sulfur containing volatile organic compound produced by plants and bacterial species, has recently been described to be an efficient anti-oomycete agent with promising perspectives for the control of the devastating potato late blight disease caused by Phytophthora infestans. However, earlier work raised questions regarding the putative...

Université de Fribourg

In vivo function of the lipid raft protein flotillin-1 during cd8+ t cell–mediated host surveillance

Ficht, Xenia ; Ruef, Nora ; Stolp, Bettina ; Samson, Guerric P. B. ; Moalli, Federica ; Page, Nicolas ; Merkler, Doron ; Nichols, Ben J. ; Diz-Muñoz, Alba ; Legler,, Daniel F. ; Niggli, Verena

In: The Journal of Immunology, 2019, p. -

Flotillin-1 (Flot1) is an evolutionary conserved, ubiquitously expressed lipid raft– associated scaffolding protein. Migration of Flot1-deficient neutrophils is impaired because of a decrease in myosin II–mediated contractility. Flot1 also accumulates in the uropod of polarized T cells, suggesting an analogous role in T cell migration. In this study, we analyzed morphology and migration...

Università della Svizzera italiana

Herpes simplex virus enhances chemokine function through modulation of receptor trafficking and oligomerization

Martinez-Martin, Nadia ; Viejo-Borbolla, Abel ; Martín, Rocío ; Soledad, Blanco ; Benovic, Jeffrey L. ; Thelen, Marcus ; Alcamí, Antonio

In: Nature communications, 2015, vol. 6, p. 6163

Glycoprotein G (gG) from herpes simplex virus 1 and 2 (HSV-1 and HSV-2, important human neurotropic pathogens) is the first viral chemokine-binding protein found to potentiate chemokine function. Here we show that gG attaches to cell surface glycosaminoglycans and induces lipid raft clustering, increasing the incorporation of CXCR4 receptors into these microdomains. gG induces conformational...

Consortium of Swiss Academic Libraries

Cholesterol Transport and Regulation in the Mammary Gland

Ontsouka, Edgar ; Albrecht, Christiane

In: Journal of Mammary Gland Biology and Neoplasia, 2014, vol. 19, no. 1, p. 43-58