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Università della Svizzera italiana

SARS-CoV-2 Ig G among HealthcareWorkers and the General Population

Milani, Gregorio P. ; Bianchetti, Mario G. ; Togni, Giuseppe ; Schoenenberger, Andreas W. ; Muggli, Franco

In: Pathogens, 2021, vol. 10, no. 4, p. 6

It is assumed that healthcare workers are at the highest risk to be infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, few data from healthcare workers who do not primarily take care of patients with SARS-CoV-2 infection support this assumption. We investigated the prevalence of immunoglobulin G (Ig G) against SARS-CoV-2 among healthcare workers who do not...

Università della Svizzera italiana

Atypical bacterial pathogens and small-vessel leukocytoclastic vasculitis of the skin in children : systematic literature review

Betti, Céline ; Camozzi, Pietro ; Gennaro, Viola ; Bianchetti, Mario G. ; Scoglio, Martin ; Simonetti, Giacomo D. ; Milani, Gregorio P. ; Lava, Sebastiano A. G. ; Ferrarini, Alessandra

In: Pathogens, 2021, vol. 10, no. 1, p. 9 p

Leukocytoclastic small-vessel vasculitis of the skin (with or without systemic involvement) is often preceded by infections such as common cold, tonsillopharyngitis, or otitis media. Our purpose was to document pediatric (≤18 years) cases preceded by a symptomatic disease caused by an atypical bacterial pathogen. We performed a literature search following the Preferred Reporting of...

Université de Fribourg

A selective culture medium for screening ceftazidime-avibactam resistance in Enterobacterales and Pseudomonas aeruginosa

Sadek, Mustafa ; Poirel, Laurent ; Tinguely, Camille ; Nordmann, Patrice

In: Journal of Clinical Microbiology, 2020, vol. 58, no. 9, p. -

The SuperCAZ/AVI medium was developed for screening ceftazidime-avibactam (CZA) resistance among Gram-negative bacteria (Enterobacterales and Pseudomonas aeruginosa). It was evaluated using 50 CZA-susceptible and 42 CZA- resistant Gram-negative isolates. Its sensitivity and specificity of detection were 100%. Excellent performance of the medium was also observed by testing spiked stools, with ...

Université de Fribourg

Covid-19 in children, pregnancy and neonates: a review of epidemiologic and clinical features

Zimmermann, Petra ; Curtis , Nigel

In: The Pediatric Infectious Disease Journal, 2020, vol. 39, no. 6, p. 469–477

The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has spread rapidly across the globe. In contrast to initial reports, recent studies suggest that children are just as likely as adults to become infected with the virus but have fewer symptoms and less severe disease. In this review, we summarize the epidemiologic and clinical features of children infected with...

Université de Fribourg

Implementation and evaluation of methods for the optimal detection of carbapenem-resistant and colistin-resistant Pseudomonas aeruginosa and Acinetobacter baumannii from stools

Fournier, Claudine ; Poirel, Laurent ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2020, vol. 98, no. 2, p. 115121

Carbapenem-resistant Acinetobacter baumannii (CRAB) and Pseudomonas aeruginosa (CRPA), as well as polymyxin-resistant A. baumannii (PMR-AB) and P. aeruginosa (PMR-PA), were used to test different enrichment strategies from spiked stools. Three procedures were compared, namely, direct inoculation on selective plates and plating after a 24-h enrichment step in tryptic soy broth with or without ...

Université de Fribourg

Pathogenicity genomic island-associated CrpP-like fluoroquinolone-modifying enzymes among Pseudomonas aeruginosa clinical isolates in Europe

Ortiz de la Rosa, José Manuel ; Nordmann, Patrice ; Poirel, Laurent

In: Antimicrobial Agents and Chemotherapy, 2020, vol. 64, no. 7, p. -

Many transferable quinolone resistance mechanisms have been identified in Gram- negative bacteria. The plasmid-encoded 65-amino-acid-long ciprofloxacin-modifying enzyme CrpP was recently identified in Pseudomonas aeruginosa isolates. We analyzed a collection of 100 clonally unrelated and multidrug-resistant P. aeruginosa clinical isolates, among which 46 were positive for crpP-like genes,...

Université de Fribourg

Rapid polymyxin/pseudomonas NP test for rapid detection of polymyxin susceptibility/resistance in Pseudomonas aeruginosa

Sadek, Mustafa ; Tinguely, Camille ; Poirel, Laurent ; Nordmann, Patrice

In: European Journal of Clinical Microbiology & Infectious Diseases, 2020, p. -

Polymyxins are becoming an added value for treating infections due to multidrug-resistant Pseudomonas aeruginosa. Therefore, the Rapid Polymyxin/Pseudomonas NP test was developed for the identification of polymyxin resistance in P. aeruginosa isolates. This test detects the bacterial growth in a medium containing a defined concentration of colistin. Formation of basic metabolites is visually...

Université de Fribourg

ESBLs and resistance to ceftazidime/avibactam and ceftolozane/tazobactam combinations in Escherichia coli and Pseudomonas aeruginosa

Ortiz de la Rosa, José-Manuel ; Nordmann, Patrice ; Poirel, Laurent

In: Journal of Antimicrobial Chemotherapy, 2019, vol. 74, no. 7, p. 1934–1939

To evaluate the efficacy of the recently launched β-lactam/β-lactamase inhibitor combinations ceftazidime/avibactam and ceftolozane/tazobactam against ESBL- producing Escherichia coli and Pseudomonas aeruginosa strains.Methods: A series of ESBL-encoding genes (blaTEM, blaSHV, blaCTX-M, blaVEB, blaPER, blaGES and blaBEL) was cloned and expressed in E. coli or P. aeruginosa recipient strains. ...

Università della Svizzera italiana

The neonatal Fc receptor (FcRn) enhances human immunodeficiency virus type 1 (HIV-1) transcytosis across epithelial cells

Gupta, Sandeep ; Gach, Johannes S. ; Becerra, Juan C. ; Phan, Tran B. ; Pudney, Jeffrey ; Moldoveanu, Zina ; Joseph, Sarah B. ; Landucci, Gary ; Supnet, Medalyn Jude ; Ping, Li-Hua ; Corti, Davide ; Moldt, Brian ; Hel, Zdenek ; Lanzavecchia, Antonio ; Ruprecht, Ruth M. ; Burton, Dennis R. ; Mestecky, Jiri ; Anderson, Deborah J. ; Forthal, Donald N.

In: Plos pathogens, 2013, vol. 9, no. 11, p. e1003776

The mechanisms by which human immunodeficiency virus type 1 (HIV-1) crosses mucosal surfaces to establish infection are unknown. Acidic genital secretions of HIV-1-infected women contain HIV-1 likely coated by antibody. We found that the combination of acidic pH and Env- specific IgG, including that from cervicovaginal and seminal fluids of HIV-1-infected individuals, augmented transcytosis...