Université de Fribourg

Shining a light on species delimitation in the tree genus Engelhardia Leschenault ex Blume (Juglandaceae)

Zhang, Can-Yu ; Low, Shook Ling ; Song, Yi-Gang ; Nuraina ; Kozlowski, Gregor ; Do, Truong Van ; Li, Lang ; Zhou, Shi-Shun ; Tan, Yun-Hong ; Cao, Guan-Long ; Zhou, Zhuo ; Meng, Hong-Hu ; Li, Jie

In: Molecular Phylogenetics and Evolution, 2020, vol. 152, p. 106918

Enhanced efficacy in species delimitation is critically important in biology given the pending biodiversity crisis under global warming and anthropogenic activity. In particular, delineation of traditional classifications in view of the complexity of species requires an integrative approach to effectively define species boundaries, and this is a major focus of systematic biology. Here, we...

Université de Fribourg

High-risk KPC-producing Klebsiella pneumoniae lack type I R-M systems

Zhou, Ying ; Tian, Dongxing ; Tang, Yu ; Yu, Lianhua ; Huang, Yunkun ; Li, Gang ; Li, Meng ; Wang, Yong ; Yang, Zehua ; Poirel, Laurent ; Jiang, Xiaofei

In: International Journal of Antimicrobial Agents, 2020, vol. 56, no. 2, p. 106050

Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP) have disseminated worldwide and are a major threat to public health. The multidrug- resistant (MDR)-phenotype of KPC-KP are commonly associated with the presence of high molecular weight blaKPC plasmids. Restriction-modification (R-M) systems provide bacteria with innate defense against plasmids or other infectious gene...

Université de Fribourg

The type I-E CRISPR-Cas system influences the acquisition of bla KPC-IncF plasmid in Klebsiella pneumonia

Zhou, Ying ; Tang, Yu ; Fu, Pan ; Tian, Dongxing ; Yu, Lianhua ; Huang, Yunkun ; Li, Gang ; Li, Meng ; Wang, Yong ; Yang, Zehua ; Xu, Xiaogang ; Yin, Zhe ; Zhou, Dongsheng ; Poirel, Laurent ; Jiang, Xiaofei

In: Emerging Microbes & Infections, 2020, vol. 9, no. 1, p. 1011–1022

Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and bla KPC-IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene ...

Université de Fribourg

Structural insights into the EGO-TC–mediated membrane tethering of the TORC1-regulatory Rag GTPases

Zhang, Tianlong ; Péli-Gulli, Marie-Pierre ; Zhang, Zhen ; Tang, Xin ; Ye, Jie ; Virgilio, Claudio De ; Ding, Jianping

In: Science Advances, 2019, vol. 5, no. 9, p. eaax8164

The Rag/Gtr GTPases serve as a central module in the nutrient-sensing signaling network upstream of TORC1. In yeast, the anchoring of Gtr1-Gtr2 to membranes depends on the Ego1-Ego2-Ego3 ternary complex (EGO-TC), resulting in an EGO- TC-Gtr1-Gtr2 complex (EGOC). EGO-TC and human Ragulator share no obvious sequence similarities and also differ in their composition with respect to the number of ...

Université de Fribourg

Measurement of ultra-trace level of intact oxytocin in plasma using SALLE combined with nano-LC–MS

Liu, Dan ; Han, Xiaoli ; Liu, Xinxin ; Cheng, Mengchun ; He, Meixi ; Rainer, Gregor ; Gao, Huiyuan ; Zhang, Xiaozhe

In: Journal of Pharmaceutical and Biomedical Analysis, 2019, vol. 173, p. 62–67

Measurement of peptides such as oxytocin in plasma is a critical challenge in clinical research because of their extreme low concentrations as well as the tremendous interferencing substances co-presented in plasma. In this study, we developed an efficient salt-out assisted liquid-liquid extraction (SALLE) to treat plasma, and then analyzed the samples using nano-LC–MS to quantify intact...

Université de Fribourg

The auxin influx carrier, OsAUX3, regulates rice root development and responses to aluminium stress

Wang, Mei ; Qiao, JiYue ; Yu, ChenLiang ; Chen, Hao ; Sun, ChenDong ; Huang, LinZhou ; Li, ChuanYou ; Geisler, Markus ; Qian, Qian ; Jiang, De An ; Qi, YanHua

In: Plant, Cell & Environment, 2019, vol. 42, no. 4, p. 1125–1138

In rice, there are five members of the auxin carrier AUXIN1/LIKE AUX1 family; however, the biological functions of the other four members besides OsAUX1 remain unknown. Here, by using CRISPR/Cas9, we constructed two independent OsAUX3 knock‐down lines, osaux3‐1 and osaux3‐2, in wild‐type rice, Hwayoung (WT/HY) and Dongjin (WT/DJ). osaux3‐1 and osaux3‐2 have shorter primary roots...