In: Current opinion in genetics & development, 2021, vol. 71, p. 39-47
DNA breaks may arise accidentally in vegetative cells or in a programmed manner in meiosis. The usage of a DNA template makes homologous recombination potentially error-free, however, recombination is not always accurate. Cells possess a remarkable capacity to tailor processing of recombination intermediates to fulfill a particular need. Vegetatively growing cells aim to maintain genome...
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In: Interactive Cardiovascular and Thoracic Surgery, ///-
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In: Human Molecular Genetics, 2018, vol. 27, no. 12, p. 2039-2051
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In: Journal of Inherited Metabolic Disease, 2015, vol. 38, no. 5, p. 863-872
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In: Cell reports, 2017, vol. 20, no. 8, p. 1921-1935
DNA double-strand break (DSB) repair by homologous recombination (HR) is initiated by CtIP/MRN-mediated DNA end resection to maintain genome integrity. SAMHD1 is a dNTP triphosphohydrolase, which restricts HIV- 1 infection, and mutations are associated with Aicardi-Goutières syndrome and cancer. We show that SAMHD1 has a dNTPase-independent function in promoting DNA end resection to facilitate...
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In: Communications Biology, 2019, vol. 2, p. 174
Dna2 is an essential nuclease-helicase that acts in several distinct DNA metabolic pathways including DNA replication and recombination. To balance these functions and prevent unscheduled DNA degradation, Dna2 activities must be regulated. Here we show that Saccharomyces cerevisiae Dna2 function is controlled by sumoylation. We map the sumoylation sites to the N-terminal regulatory domain of...
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In: Molecular Cell, 2017, vol. 68, no. 2, p. 414-430.e8
To ensure the completion of DNA replication and maintenance of genome integrity, DNA repair factors protect stalled replication forks upon replication stress. Previous studies have identified a critical role for the tumor suppressors BRCA1 and BRCA2 in preventing the degradation of nascent DNA by the MRE11 nuclease after replication stress. Here we show that depletion of SMARCAL1, a...
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In: International Journal of Colorectal Disease, 2014, vol. 29, no. 6, p. 663-671
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In: Genes and Development, 2017, vol. 31, no. 5, p. 493-502
DNA2 nuclease–helicase functions in DNA replication and recombination. This requires the nuclease of DNA2, while, in contrast, the role of the helicase activity has been unclear. We now show that the motor activity of both recombinant yeast and human DNA2 promotes efficient degradation of long stretches of ssDNA, particularly in the presence of the replication protein A. This degradation is...
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In: Human Genetics, 2005, vol. 116, no. 6, p. 461-465
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