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Université de Fribourg

Efficacy of dual carbapenem treatment in a murine sepsis model of infection due to carbapenemase-producing Acinetobacter baumannii

Cebrero-Cangueiro, T. ; Nordmann, Patrice ; Carretero-Ledesma, M. ; Pachón, J. ; Pachón-Ibáñez, M. E.

In: Journal of Antimicrobial Chemotherapy, 2020, p. dkaa487

Objectives: To evaluate the in vivo efficacy of a dual carbapenem combination containing imipenem plus meropenem against carbapenem-resistant Acinetobacter baumannii producing carbapenemases OXA-23 or OXA-58.Methods: An experimental model of peritonitis using C57BL/6J female mice was developed and the minimum lethal doses were calculated for infections due to OXA-23 or OXA-58 producers of A. ...

Université de Fribourg

NGS-based S. aureus typing and outbreak analysis in clinical microbiology laboratories : Lessons learned from a Swiss-wide proficiency test

Dylus, David ; Pillonel, Trestan ; Opota, Onya ; Wüthrich, Daniel ; Seth-Smith, Helena M. B. ; Egli, Adrian ; Leo, Stefano ; Lazarevic, Vladimir ; Schrenzel, Jacques ; Laurent, Sacha ; Bertelli, Claire ; Blanc, Dominique S. ; Neuenschwander, Stefan ; Ramette, Alban ; Falquet, Laurent ; Imkamp, Frank ; Keller, Peter M. ; Kahles, Andre ; Oberhaensli, Simone ; Barbié, Valérie ; Dessimoz, Christophe ; Greub, Gilbert ; Lebrand, Aitana

In: Frontiers in Microbiology, 2020, vol. 11, p. 591093

Whole genome sequencing (WGS) enables high resolution typing of bacteria up to the single nucleotide polymorphism (SNP) level. WGS is used in clinical microbiology laboratories for infection control, molecular surveillance and outbreak analyses. Given the large palette of WGS reagents and bioinformatics tools, the Swiss clinical bacteriology community decided to conduct a ring trial (RT) to...

Université de Fribourg

Whole-genome sequence of a colombian Acinetobacter baumannii strain, a coproducer of OXA-72 and OXA-255-like carbapenemases

Saavedra, Sandra Yamile ; Prada-Cardozo, Diego ; Rincón, Verónica ; Pérez-Cardona, Hermes ; Hidalgo, Andrea Melissa ; González, María Nilse ; Reguero, María T. ; Silva, Emilia M. Valenzuela de ; Mantilla, José R. ; Falquet, Laurent ; Barreto-Hernández, Emiliano ; Duarte, Carolina

In: Genome Announcements, 2017, vol. 5, no. 7, p. e01558-16

Colombian Acinetobacter baumannii strain ST920 was isolated from the sputum of a 68-year-old male patient. This isolate possessed blaOXA-72 and blaOXA-255-like genes. The assembled genome contained 4,104,098 pb and 38.79% G+C content. This is the first case reported of the coproduction (blaOXA-72 and blaOXA-255-like) of carbapenem-hydrolyzing class D β-lactamases (CHDLs) in Acinetobacter...

Université de Fribourg

Draft genome sequences of multidrug-resistant acinetobacter sp. strains from colombian hospitals

Barreto-Hernández, Emiliano ; Falquet, Laurent ; Reguero, María T. ; Mantilla, José R. ; Valenzuela, Emilia M. ; González, Elsa ; Cepeda, Alexandra ; Escalante, Andrea

In: Genome Announcements, 2013, vol. 1, no. 6, p. -

The draft genome sequences of the strains Acinetobacter baumannii 107m, Acinetobacter nosocomialis 28F, and Acinetobacter pittii 42F, isolated from Colombian hospitals, are reported here. These isolates are causative of nosocomial infections and are classified as multidrug resistant, as they showed resistance to four different antibiotic groups.

Université de Fribourg

Implementation and evaluation of methods for the optimal detection of carbapenem-resistant and colistin-resistant Pseudomonas aeruginosa and Acinetobacter baumannii from stools

Fournier, Claudine ; Poirel, Laurent ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2020, vol. 98, no. 2, p. 115121

Carbapenem-resistant Acinetobacter baumannii (CRAB) and Pseudomonas aeruginosa (CRPA), as well as polymyxin-resistant A. baumannii (PMR-AB) and P. aeruginosa (PMR-PA), were used to test different enrichment strategies from spiked stools. Three procedures were compared, namely, direct inoculation on selective plates and plating after a 24-h enrichment step in tryptic soy broth with or without ...

Université de Fribourg

Wide spread of carbapenemase-producing bacterial isolates in a Nigerian environment

Terrier, Christophe Le ; Masseron, Amandine ; Uwaezuoke, Nkolika Stella ; P.Edwin, Chinagozi ; E.Ekuma, Agantem ; Olugbeminiyi, Folake ; Shettima, Shuwaram ; Ushie, Simon ; Poirel, Laurent ; Nordmann, Patrice

In: Journal of Global Antimicrobial Resistance, 2020, vol. 21, p. 321–323

Objectives: The presence of carbapenemase-producing bacterial isolates is found not only in hospital and community settings but also in the environment. Carbapenemase production may be related to acquired, usually plasmid-borne, β-lactamase genes or to chromosomal genes intrinsic to various species. The aim of this study was to evaluate the occurrence of such carbapenemase-producing...

Université de Fribourg

Evaluation of resazurin-based rapid test to detect colistin resistance in Acinetobacter baumannii isolates

Germ, Julija ; Poirel, Laurent ; Cerar Kisek, Tjasa ; Cvitkovic Spik, Vesna ; Katja Seme ; Mueller Premru, Manica ; Lejko Zupanc, Tatjana ; Nordmann, Patrice ; Pirs, Mateja

In: European Journal of Clinical Microbiology & Infectious Diseases, 2019, vol. 38, no. 11, p. 2159–2162

Acinetobacter baumannii primarily causes colonization, yet it can be an opportunistic pathogen associated with hospital-acquired infections. Many countries report rapid spread of carbapenem-resistant Acinetobacter baumannii (CRAb) which limits treatment options, with colistin frequently being the last line treatment option. The aim of our study was to evaluate a recently developed rapid...

Université de Fribourg

A resazurin reduction-based assay for rapid detection of polymyxin resistance in Acinetobacter baumannii and Pseudomonas aeruginosa

Lescat, Mathilde ; Poirel, Laurent ; Tinguely, Camille ; Nordmann, Patrice

In: Journal of Clinical Microbiology, 2018, p. -

A rapid test was developed for identification of polymyxin resistance in non-fermenting bacteria. This test detects viable cells after growth in a medium containing a defined concentration of colistin. The principle of this test is based on the visual detection of the reduction of the resazurin reagent, a viability colorant, observed by its color change (blue to purple or pink). Its...