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Acyl-CoA-binding protein, Acb1p, is required for normal vacuole function and ceramide synthesis in Saccharomyces cerevisiae

Faergeman, Nils J. ; Feddersen, Søren ; Christiansen, Janne K. ; Larsen, Morten K. ; Schneiter, Roger ; Ungermann, Christian ; Mutenda, Kudzai ; Roepstroff, Peter ; Knudsen, Jens

In: Biochemical Journal, 2004, vol. 380, p. 907-918

In the present study, we show that depletion of acyl-CoA-binding protein, Acb1p, in yeast affects ceramide levels, protein trafficking, vacuole fusion and structure. Vacuoles in Acb1p-depleted cells are multi-lobed, contain significantly less of the SNAREs (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptors) Nyv1p, Vam3p and Vti1p, and are unable to fuse in vitro. Mass...