Université de Fribourg

Elongation factor 1a mediates the specificity of mitochondrial tRNA import in T. brucei

Bouzaidi-Tiali, Nabile ; Aeby, Eric ; Charrière, Fabien ; Pusnik, Mascha ; Schneider, André

In: The EMBO Journal, 2007, vol. 26, no. 20, p. 4302–4312

Mitochondrial tRNA import is widespread in eukaryotes. Yet, the mechanism that determines its specificity is unknown. Previous in vivo experiments using the tRNAsMet, tRNAIle and tRNALys have suggested that the T-stem nucleotide pair 51:63 is the main localization determinant of tRNAs in Trypanosoma brucei. In the cytosol-specific initiator...