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Université de Fribourg

Reactive oxygen species and plant resistance to fungal pathogens

Lehmann, Silke ; Serrano, Mario ; L’Haridon, Floriane ; Tjamos, Sotirios E. ; Metraux, Jean-Pierre

In: Phytochemistry, 2015, vol. 112, p. 54–62

Reactive oxygen species (ROS) have been studied for their role in plant development as well as in plant immunity. ROS were consistently observed to accumulate in the plant after the perception of pathogens and microbes and over the years, ROS were postulated to be an integral part of the defence response of the plant. In this article we will focus on recent findings about ROS involved in the...

Université de Fribourg

Transcriptome structure variability in Saccharomyces cerevisiae strains determined with a newly developed assembly software

Sardu, Alessandro ; Treu, Laura ; Campanaro, Stefano

In: BMC Genomics, 2014, vol. 15, no. 1, p. 1045

RNA-seq studies have an important role for both large-scale analysis of gene expression and for transcriptome reconstruction. However, the lack of software specifically developed for the analysis of the transcriptome structure in lower eukaryotes, has so far limited the comparative studies among different species and strains.Results: In order to fill this gap, an innovative software called ORA...

Université de Fribourg

Biosynthesis and function of GPI proteins in the yeast Saccharomyces cerevisiae

Pittet, Martine ; Conzelmann, Andreas

In: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2007, vol. 1771, no. 3, p. 405-420

Like most other eukaryotes, Saccharomyces cerevisiae harbors a GPI anchoring machinery and uses it to attach proteins to membranes. While a few GPI proteins reside permanently at the plasma membrane, a majority of them gets further processed and is integrated into the cell wall by a covalent attachment to cell wall glucans. The GPI biosynthetic pathway is necessary for growth and survival...

Université de Fribourg

Induction of (1→3,1→4)-ß-D-Glucan hydrolases in leaves of dark-incubated barley seedlings

Roulin, Samuel ; Buchala, Antony J. ; Fincher, Geoffrey B.

In: Planta, 2002, vol. 215, no. 1, p. 51-59

When seedlings of barley (Hordeum vulgare L.) were transferred from a natural light/dark cycle into darkness, (1M3,1M4)-#-D-glucan endohydrolase (EC 3.2.1.73) activity in leaf extracts increased 3- to 4-fold after 2 days. Activity decreased to normal levels within a day if the light/dark cycle was restored. Although there are two (1M3,1M4)-#-D-glucan endohydrolase...