Search Results - RERO DOC

Bridging the gap between qualitative and quantitative colocalization results in fluorescence microscopy studies

Zinchuk, Vadim ; Wu, Yong ; Grossenbacher-Zinchuk, Olga

In: Scientific Reports, 2013, vol. 3, p. 1365

Quantitative colocalization studies suffer from the lack of unified approach to interpret obtained results. We developed a tool to characterize the results of colocalization experiments in a way so that they are understandable and comparable both qualitatively and quantitatively. Employing a fuzzy system model and computer simulation, we produced a set of just five linguistic variables tied to...

OPEN IN
SONAR

Quantifying spatial correlations of fluorescent markers using enhanced background reduction with protein proximity index and correlation coefficient estimations

Zinchuk, Vadim ; Wu, Yong ; Grossenbacher-Zinchuk, Olga ; Stefani, Enrico

In: Nature Protocols, 2011, vol. 6, p. 1554–1567

Interactions of proteins are examined by detecting their overlap using fluorescent markers. The observed overlap is then quantified to serve as a measure of spatial correlation. A major drawback of this approach is that it can produce false values because of the properties of the image background. To remedy this, we provide a protocol to reduce the contribution of image background and then apply...

Refine my results

Document type

Institution

Specific Collection

Language

Author