Affiner les résultats

High-efficiency recognition and identification of disulfide bonded peptides in rat neuropeptidome using targeted electron transfer dissociation tandem mass spectrometry

Yu, Xi ; Khani, Abbas ; Ye, Xueting ; Petruzziello, Filomena ; Gao, Huiyuan ; Zhang, Xiaozhe ; Rainer, Gregor

In: Analytical Chemistry, 2015, vol. 87, no. 23, p. 11646–11651

The main goal of the present study is to develop a method to recognize and identify endogenous intrachain disulfide bonded peptide, which are rarely sequenced in current peptidomics studies. In order to achieve highly efficient detection of these peptides in a neuropeptidome analysis, we alkylated the peptides, mined the raw mass spectrometry data, and then recognized the candidates of untreated...