Université de Fribourg

A selective culture medium for screening ceftazidime-avibactam resistance in Enterobacterales and Pseudomonas aeruginosa

Sadek, Mustafa ; Poirel, Laurent ; Tinguely, Camille ; Nordmann, Patrice

In: Journal of Clinical Microbiology, 2020, vol. 58, no. 9, p. -

The SuperCAZ/AVI medium was developed for screening ceftazidime-avibactam (CZA) resistance among Gram-negative bacteria (Enterobacterales and Pseudomonas aeruginosa). It was evaluated using 50 CZA-susceptible and 42 CZA- resistant Gram-negative isolates. Its sensitivity and specificity of detection were 100%. Excellent performance of the medium was also observed by testing spiked stools, with ...

Université de Fribourg

Rapid polymyxin/pseudomonas NP test for rapid detection of polymyxin susceptibility/resistance in Pseudomonas aeruginosa

Sadek, Mustafa ; Tinguely, Camille ; Poirel, Laurent ; Nordmann, Patrice

In: European Journal of Clinical Microbiology & Infectious Diseases, 2020, p. -

Polymyxins are becoming an added value for treating infections due to multidrug-resistant Pseudomonas aeruginosa. Therefore, the Rapid Polymyxin/Pseudomonas NP test was developed for the identification of polymyxin resistance in P. aeruginosa isolates. This test detects the bacterial growth in a medium containing a defined concentration of colistin. Formation of basic metabolites is visually...

Université de Fribourg

A resazurin reduction-based assay for rapid detection of polymyxin resistance in Acinetobacter baumannii and Pseudomonas aeruginosa

Lescat, Mathilde ; Poirel, Laurent ; Tinguely, Camille ; Nordmann, Patrice

In: Journal of Clinical Microbiology, 2018, p. -

A rapid test was developed for identification of polymyxin resistance in non-fermenting bacteria. This test detects viable cells after growth in a medium containing a defined concentration of colistin. The principle of this test is based on the visual detection of the reduction of the resazurin reagent, a viability colorant, observed by its color change (blue to purple or pink). Its...