Université de Fribourg

Fungicide-driven evolution and molecular basis of multidrug resistance in field populations of the grey mould fungus Botrytis cinerea

Kretschmer, Matthias ; Leroch, Michaela ; Mosbach, Andreas ; Walker, Anne-Sophie ; Fillinger, Sabine ; Mernke, Dennis ; Schoonbeek, Henk-jan ; Pradier, Jean-Marc ; Leroux, Pierre ; Waard, Maarten A. De ; Hahn, Matthias

In: PLoS Pathogens, 2009, vol. 5, no. 12, p. e1000696

The grey mould fungus Botrytis cinerea causes losses of commercially important fruits, vegetables and ornamentals worldwide. Fungicide treatments are effective for disease control, but bear the risk of resistance development. The major resistance mechanism in fungi is target protein modification resulting in reduced drug binding. Multiple drug resistance (MDR) caused by increased efflux activity...

Université de Fribourg

The ABC transporter BcatrB from Botrytis cinerea exports camalexin and is a virulence factor on Arabidopsis thaliana

Stefanato, Francesca L. ; Abou-Mansour, Eliane ; Buchala, Antony ; Kretschmer, Matthias ; Mosbach, Andreas ; Hahn, Matthias ; Bochet, Christian G. ; Métraux, Jean-Pierre ; Schoonbeek, Henk-jan

In: The Plant Journal, 2009///doi: 10.1111/j.1365-313X.2009.03794.x

Arabidopsis thaliana is known to produce the phytoalexin camalexin in response to abiotic and biotic stress. Here we studied the mechanisms of tolerance to camalexin in the fungus Botrytis cinerea, a necrotrophic pathogen of A. thaliana. Exposure of B. cinerea to camalexin induces expression of BcatrB, an ABC transporter that functions in the efflux of fungitoxic compounds. B. cinerea inoculated...

Université de Fribourg

Wounding of Arabidopsis leaves causes a powerful but transient protection against Botrytis infection

Chassot, Céline ; Buchala, Antony ; Schoonbeek, Henk-jan ; Métraux, Jean-Pierre ; Lamotte, Olivier

In: The Plant Journal, 2008///doi:10.1111/j.1365-313X.2008.03540.x

Physical injury inflicted on living tissue makes it vulnerable to invasion by pathogens. Wounding of Arabidopsis thaliana leaves, however, does not conform to this concept and leads to immunity to Botrytis cinerea, the causal agent of grey mould. In wounded leaves, hyphal growth was strongly inhibited compared to unwounded controls. Wound-induced resistance was not associated with salicylic...