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In vitro imaging of primary neural cell culture from Drosophila

Egger, Boris ; Giesen, Lena van ; Moraru, Manuela ; Sprecher, Simon G.

In: Nature Protocols, 2013, vol. 8, no. 5, p. 958–965

Cell culture systems are widely used for molecular, genetic and biochemical studies. Primary cell cultures of animal tissues offer the advantage that specific cell types can be studied in vitro outside of their normal environment. We provide a detailed protocol for generating primary neural cell cultures derived from larval brains of Drosophila melanogaster. The developing larval brain contains...