Université de Fribourg

Mature lipid droplets are accessible to ER luminal proteins

Mishra, Shirish ; Khaddaj, Rasha ; Cottier, Stéphanie ; Stradalova, Vendula ; Jacob, Claire ; Schneiter, Roger

In: Journal of Cell Science, 2016, vol. 129, no. 20, p. 3803–3815

Lipid droplets are found in most organisms where they serve to store energy in the form of neutral lipids. They are formed at the endoplasmic reticulum (ER) membrane where the neutral-lipid-synthesizing enzymes are located. Recent results indicate that lipid droplets remain functionally connected to the ER membrane in yeast and mammalian cells to allow the exchange of both lipids and integral...

Université de Fribourg

Expression of oleosin and perilipins in yeast promote formation of lipid droplets from the endoplasmatic reticulum

Jacquier, Nicolas ; Mishra, Shirish ; Choudhary, Vineet ; Schneiter, Roger

In: Journal of Cell Science, 2013, p. -

Most cells store neutral lipids in a dedicated compartment, the lipid droplet (LD). These LDs are structurally and functionally conserved across species. In higher eukaryotes, LDs are covered by abundant scaffolding proteins, such as the oleosins in plants and perilipins (PLINs) in animal cells. S. cerevisiae, however, has no homologues of these scaffolding proteins. To analyze a possible...