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Functional characterization of a miniature inverted transposable element at the origin of mcr-5 gene acquisition in Escherichia coli

Kieffer, Nicolas ; Nordmann, Patrice ; Millemann, Yves ; Poirel, Laurent

In: Antimicrobial Agents and Chemotherapy, 2019/63/7/e00559-19, /aac/63/7/AAC.00559-19.atom

Plasmid-mediated colistin resistance of the mobile colistin resistance (MCR) type is a growing concern in Enterobacteriaceae since it has been described worldwide in humans and animals. Here, we identified a series of MCR-producing Escherichia coli isolates corresponding to two different clones (represented by isolates PS1 and PS8b) producing MCR-1 and MCR-5, respectively, obtained from pig...

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IncH-type plasmid harboring the blaCTX-M-15, blaDHA-1, and qnrB4 genes recovered from animal isolates

Schlüter, Andreas ; Nordmann, Patrice ; Bonnin, Rémy A. ; Millemann, Yves ; Eikmeyer, Felix G. ; Wibberg, Daniel ; Pühler, Alfred ; Poirel, Laurent

In: Antimicrobial Agents and Chemotherapy, 2014, p. AAC.02695–14

The whole sequence of plasmid pENVA carrying the extended-spectrum ß-lactamase gene blaCTX-M-15 was determined. It has been identified from a series of clonally-related Klebsiella pneumoniae ST274 strains recovered from companion animals. This plasmid was 253,984-bp in-size and harbored, in addition to blaCTX-M-15, a large array of genes encoding...

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