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A resazurin reduction-based assay for rapid detection of polymyxin resistance in Acinetobacter baumannii and Pseudomonas aeruginosa

Lescat, Mathilde ; Poirel, Laurent ; Tinguely, Camille ; Nordmann, Patrice

In: Journal of Clinical Microbiology, 2018, p. -

A rapid test was developed for identification of polymyxin resistance in non-fermenting bacteria. This test detects viable cells after growth in a medium containing a defined concentration of colistin. The principle of this test is based on the visual detection of the reduction of the resazurin reagent, a viability colorant, observed by its color change (blue to purple or pink). Its...

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Performances of the rapid polymyxin Acinetobacter and Pseudomonas tests for colistin susceptibility testing

Lescat, Mathilde ; Poirel, Laurent ; Jayol, Aurélie ; Nordmann, Patrice

In: Microbial Drug Resistance, 2018, p. -

Owing to the emergence of colistin resistance in nonfermenting Gram negative bacteria, reliable and rapid techniques for testing colistin susceptibility are needed. We evaluated the performances of the Rapid Polymyxin Acinetobacter and Pseudomonas tests using a collection of Acinetobacter baumannii and Pseudomonas aeruginosa clinical isolates.Methods: Colistin susceptibility of A. baumannii...

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