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Effects of the Protein Kinase Inhibitor PKC412 on Gene Expression and Link to Physiological Effects in Zebrafish Danio rerio Eleuthero-Embryos

Oggier, Daniela M. ; Lenard, Anna ; Küry, Michael ; Hoeger, Birgit ; Affolter, Markus ; Fent, Karl

In: Toxicological Sciences, 2011, vol. 119, no. 1, p. 104-115

Protein interference applications in cellular and developmental biology using DARPins that recognize GFP and mCherry

Brauchle, Michael ; Hansen, Simon ; Caussinus, Emmanuel ; Lenard, Anna ; Ochoa-Espinosa, Amanda ; Scholz, Oliver ; Sprecher, Simon G. ; Plückthun, Andreas ; Affolter, Markus

In: Biology Open, 2014, vol. 3, no. 12, p. 1252–1261

Protein–protein interactions are crucial for cellular homeostasis and play important roles in the dynamic execution of biological processes. While antibodies represent a well-established tool to study protein interactions of extracellular domains and secreted proteins, as well as in fixed and permeabilized cells, they usually cannot be functionally expressed in the cytoplasm of living cells....