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3D imaging of undissected optically cleared Anopheles stephensi mosquitoes and midguts infected with Plasmodium parasites

Niz, Mariana De ; Kehrer, Jessica ; Brancucci, Nicolas M. B. ; Moalli, Federica ; Reynaud, Emmanuel G. ; Stein, Jens V. ; Frischknecht, Friedrich

In: PLOS ONE, 2020, vol. 15, no. 9, p. e0238134

Malaria is a life-threatening disease, caused by Apicomplexan parasites of the Plasmodium genus. The Anopheles mosquito is necessary for the sexual replication of these parasites and for their transmission to vertebrate hosts, including humans. Imaging of the parasite within the insect vector has been attempted using multiple microscopy methods, most of which are hampered by the presence of...