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Suppressor mutations in Rpf2–Rrs1 or Rpl5 bypass the Cgr1 function for pre-ribosomal 5S RNP-rotation

Thoms, Matthias ; Mitterer, Valentin ; Kater, Lukas ; Falquet, Laurent ; Beckmann, Roland ; Kressler, Dieter ; Hurt, Ed

In: Nature Communications, 2018, vol. 9, no. 1, p. 4094

During eukaryotic 60S biogenesis, the 5S RNP requires a large rotational movement to achieve its mature position. Cryo-EM of the Rix1-Rea1 pre-60S particle has revealed the post-rotation stage, in which a gently undulating α-helix corresponding to Cgr1 becomes wedged between Rsa4 and the relocated 5S RNP, but the purpose of this insertion was unknown. Here, we show that cgr1 deletion in...