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Complementation of the embryo-lethal T-DNA insertion mutant of AUXIN-BINDING-PROTEIN 1 (ABP1) with abp1 point mutated versions reveals crosstalk of ABP1 and phytochromes

Effendi, Yunus ; Ferro, Noel ; Labusch, Corinna ; Geisler, Markus ; Scherer, Günther F. E.

In: Journal Of Experimental Botany, 2015, vol. 66, no. 1, p. 403-418

Complementation of the embryo-lethal T-DNA insertion mutant of AUXIN-BINDING-PROTEIN 1 (ABP1) with abp1 point mutated versions reveals crosstalk of ABP1 and phytochromes

Effendi, Yunus ; Ferro, Noel ; Labusch, Corinna ; Geisler, Markus ; Scherer, Günther F. E.

In: Journal of Experimental Botany, 2015, vol. 66, no. 1, p. 403-418

The function of the extracytoplasmic AUXIN-BINDING-PROTEIN1 (ABP1) is largely enigmatic. We complemented a homozygous T-DNA insertion null mutant of ABP1 in Arabidopsis thaliana Wassilewskia with three mutated and one wild-type (wt) ABP1 cDNA, all tagged C-terminally with a strepII–FLAG tag upstream the KDEL signal. Based on in silico modelling, the abp1 mutants were predicted to have altered...