Université de Fribourg

Rapid ESBL NP test for rapid detection of expanded-spectrum β-lactamase producers in enterobacterales

Demord, Anthony ; Poirel, Laurent ; D'Emidio, Fernando ; Pomponio, Stefano ; Nordmann, Patrice

In: Microbial Drug Resistance, 2020, p. -

Objectives: To evaluate the biochemical Rapid ESBL NP® (Liofilchem, Italy) for the rapid detection of extended-spectrum β-lactamases (ESBLs) in Enterobacterales.Methods: A total of 100 clinical gram-negative strains (40 ESBL producers with or without cephalosporinase, 43 carbapenemase producers with or without additional ESBLs, 8 AmpC-type producers, 6 penicillinase producers, and 3 ...

Université de Fribourg

Fast and reliable detection of carbapenemase genes in various Gram negatives using a new commercially available fluorescence-based real-time polymerase chain reaction platform

Sadek, Mustafa ; Demord, Anthony ; Poirel, Laurent ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2020, vol. 98, no. 3, p. 115127

The Revogene® Carba C assay is a real-time polymerase chain reaction–based assay that runs on the microfluidic Revogene platform. It was recently designed for the detection of genes encoding the 5 major carbapenemases (NDM, VIM, IMP, KPC, and OXA-48) from various Gram-negatives. A total of 145 clinical Gram-negative strains (96 carbapenemase producers and 49 non–carbapenemase producers)...

Université de Fribourg

NitroSpeed-carba NP test for rapid detection and differentiation between different classes of carbapenemases in Enterobacterales

Nordmann, Patrice ; Sadek, Mustafa ; Demord, Anthony ; Poirel, Laurent

In: Journal of Clinical Microbiology, 2020, vol. 58, no. 9, p. -

A biochemical test (NitroSpeed-Carba NP test) was developed to identify carbapenemase production in Enterobacterales and to discriminate between the different types of clinically significant carbapenemases (Ambler classes A, B, and D). It is based on two main features, namely, the hydrolysis by all β-lactamases, including carbapenemases of the nitrocefin substrate, and the capacity of...