In: Journal of Electron Microscopy, 2004, vol. 53, no. 6, p. 671-675
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In: Microscopy and Microanalysis, 2005, vol. 11(1), p. 42
Osmium postfixation is established as a routine procedure for transmission electron microscopy (TEM). On the one hand, this routine procedure leads to good results for TEM, but on the other hand results in blackened tissue samples that do not allow examination of any structures within the embedded tissue sample by a light microscope. Equivalent fixation results for TEM are achieved with...
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In: Microscopy Research and Technique, 2004, vol. 63(3), p. 149
Perisynaptic Schwann cells (PSCs), descendants of the myelinating Schwann cells, cover the axon terminal of the vertebrate motor endplate of the skeletal muscle fiber. PSCs are assumed to support the function of the axon terminal. This function suggests a net material transport in the direction of the axon terminal. Morphologically it is to be expected that these cells have a cytoskeleton aligned...
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In: Microscopy Research and Technique, 2004, vol. 63(2), p. 129
The osmium postfixation of tissue leads to good results for transmission electron microscopy, but also produces completely blackened tissue samples that do not allow the recognition of internal structures. With imidazole-osmium postfixation, one achieves comparable results in high electron microscopic resolution as with routine osmium postfixation. But the tissue samples are not blackened and...
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In: Microscopy Research and Technique, 2003, vol. 61, p. 419
By means of thin (≤150 nm) and thick (>150 nm) sections, the shape and position of the sarcoplasmic reticulum and of the Golgi apparatus in the sole plate and in the remaining subsarcolemmal sarcoplasmic region were investigated. For this purpose the membranes were stained by means of imidazole-osmium postfixation and unstained sections analyzed under the electron microscope. Both in the...
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In: Tissue and Cell, 2004, vol. 36, no. 4, p. 249-252
In the present investigation the sole plate area of motor end plates of the frog is ultrastructurally examined with different postfixation methods. We concentrated in this case on the proof of the smooth and rough sarcoplasmic reticulum of the sole plate. The relations of the smooth and rough sarcoplasmic reticulum to subsynaptic folds and the local T-system and its connections to diads and...
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In: Tissue and Cell, 2004, vol. 36 (4), p. 245-248
Previous investigations of the T-system in skeletal muscle fibres described the inter-myofibrillar relationships between T-tubules and the sarcoplasmic reticulum. They disregarded the arrangement of the T-system in the myofibril-free sarcoplasm in the area of muscle fibre nuclei. In the present investigation, the T-system was filled by means of lanthanum incubation and the myofibril-free...
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