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Symportin 1 chaperones 5S RNP assembly during ribosome biogenesis by occupying an essential rRNA-binding site

Calviño, Fabiola R. ; Kharde, Satyavati ; Ori, Alessandro ; Hendricks, Astrid ; Wild, Klemens ; Kressler, Dieter ; Bange, Gert ; Hurt, Ed ; Beck, Martin ; Sinning, Irmgard

In: Nature Communications, 2015, vol. 6, p. -

During 60S biogenesis, mature 5S RNP consisting of 5S RNA, RpL5 and RpL11, assembles into a pre-60S particle, where docking relies on RpL11 interacting with helix 84 (H84) of the 25S RNA. How 5S RNP is assembled for recruitment into the pre-60S is not known. Here we report the crystal structure of a ternary symportin Syo1–RpL5-N–RpL11 complex and provide biochemical and structural insights...