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High-level transgene expression by homologous recombination-mediated gene transfer

Grandjean, Mélanie ; Girod, Pierre-Alain ; Calabrese, David ; Kostyrko, Kaja ; Wicht, Marianne ; Yerly, Florence ; Mazza, Christian ; Beckmann, Jacques S. ; Martinet, Danielle ; Mermod, Nicolas

In: Nucleic Acids Research, 2011, vol. 39, no. 15, p. e104-e104

High-level transgene expression by homologous recombination-mediated gene transfer

Grandjean, Mélanie ; Girod, Pierre-Alain ; Calabrese, David ; Kostyrko, Kaja ; Wicht, Marianne ; Yerly, Florence ; Mazza, Christian ; Beckmann, Jacques S. ; Martinet, Danielle ; Mermod, Nicolas

In: Nucleic Acid Research, 2011, vol. 39, no. 15, p. e104

Gene transfer and expression in eukaryotes is often limited by a number of stably maintained gene copies and by epigenetic silencing effects. Silencing may be limited by the use of epigenetic regulatory sequences such as matrix attachment regions (MAR). Here, we show that successive transfections of MAR-containing vectors allow a synergistic increase of transgene expression. This finding is...