Université de Fribourg

Distance dependence of single-molecule energy transfer to graphene measured with DNA origami nanopositioners

Kaminska, I. ; Bohlen, J. ; Rocchetti, S. ; Selbach, F. ; Acuna, Guillermo P. ; Tinnefeld, P.

In: Nano Letters, 2019, vol. 19, no. 7, p. 4257–4262

Despite the thorough investigation of graphene since 2004, altering its surface chemistry and reproducible functionalization remain challenging. This hinders fabrication of more complex hybrid materials with controlled architectures, and as a consequence the development of sensitive and reliable sensors and biological assays. In this contribution, we introduce DNA origami structures as...

Université de Fribourg

Plasmon-assisted Förster resonance energy transfer at the single-molecule level in the moderate quenching regime

Bohlen, J. ; Cuartero-González, Á. ; Pibiri, E. ; Ruhlandt, D. ; Fernández-Domínguez, A. I. ; Tinnefeld, P. ; Acuna, Guillermo P.

In: Nanoscale, 2019, vol. 11, no. 16, p. 7674–7681

Metallic nanoparticles were shown to affect Förster energy transfer between fluorophore pairs. However, to date, the net plasmonic effect on FRET is still under dispute, with experiments showing efficiency enhancement and reduction. This controversy is due to the challenges involved in the precise positioning of FRET pairs in the near field of a metallic nanostructure, as well as in the...