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Université de Fribourg

SHADE AVOIDANCE 4 is required for proper auxin distribution in the hypocotyl

Ge, Yanhua ; Yan, Fenglian ; Zourelidou, Melina ; Wang, Meiling ; Ljung, Karin ; Fastner, Astrid ; Hammes, Ulrich Z. ; Donato, Martin Di ; Geisler, Markus ; Schwechheimer, Claus ; Tao, Yi

In: Plant Physiology, 2017, vol. 173, no. 1, p. 788–800

The phytohormone auxin is involved in virtually every aspect of plant growth and development. Through polar auxin transport, auxin gradients can be established, which then direct plant differentiation and growth. Shade avoidance responses are well- known processes that require polar auxin transport. In this study, we have identified a mutant, shade avoidance 4 (sav4), defective in...

Université de Fribourg

Mathematical modeling of the dynamics of shoot-root interactions and resource partitioning in plant growth

Feller, Chrystel ; Favre, Patrick ; Janka, Ales ; Zeeman, Samuel C. ; Gabriel, Jean-Pierre ; Reinhardt, Didier

In: PLoS ONE, 2015, vol. 10, no. 7, p. e0127905

Plants are highly plastic in their potential to adapt to changing environmental conditions. For example, they can selectively promote the relative growth of the root and the shoot in response to limiting supply of mineral nutrients and light, respectively, a phenomenon that is referred to as balanced growth or functional equilibrium. To gain insight into the regulatory network that controls this...

Université de Fribourg

The auxin transporter, OsAUX1, is involved in primary root and root hair elongation and in Cd stress responses in rice (Oryza sativa L.)

Yu, ChenLiang ; Sun, ChenDong ; Shen, Chenjia ; Wang, Suikang ; Liu, Fang ; Liu, Yan ; Chen, YunLong ; Li, Chuanyou ; Qian, Qian ; Aryal, Bibek ; Geisler, Markus ; Jiang, De An ; Qi, YanHua

In: The Plant Journal, 2015, vol. 83, no. 5, p. 818–830

Auxin and cadmium (Cd) stress play critical roles during root development. There are only a few reports on the mechanisms by which Cd stress influences auxin homeostasis and affects primary root (PR) and lateral root (LR) development, and almost nothing is known about how auxin and Cd interfere with root hair (RH) development. Here, we characterize rice osaux1 mutants that have a longer PR and...

Université de Fribourg

Complementation of the embryo-lethal T-DNA insertion mutant of AUXIN-BINDING-PROTEIN 1 (ABP1) with abp1 point mutated versions reveals crosstalk of ABP1 and phytochromes

Effendi, Yunus ; Ferro, Noel ; Labusch, Corinna ; Geisler, Markus ; Scherer, Günther F. E.

In: Journal of Experimental Botany, 2015, vol. 66, no. 1, p. 403-418

The function of the extracytoplasmic AUXIN-BINDING-PROTEIN1 (ABP1) is largely enigmatic. We complemented a homozygous T-DNA insertion null mutant of ABP1 in Arabidopsis thaliana Wassilewskia with three mutated and one wild-type (wt) ABP1 cDNA, all tagged C-terminally with a strepII–FLAG tag upstream the KDEL signal. Based on in silico modelling, the abp1 mutants were predicted to have altered...

Université de Fribourg

Induced jasmonate signaling leads to contrasting effects on root damage and herbivore performance

Lu, Jing ; Robert, Christelle Aurélie Maud ; Riemann, Michael ; Cosme, Marco ; Mène-Saffrané, Laurent ; Massana, Josep ; Stout, Michael Joseph ; Lou, Yonggen ; Gershenzon, Jonathan ; Erb, Matthias

In: Plant Physiology, 2015, vol. 167, no. 3, p. 1100–1116

Induced defenses play a key role in plant resistance against leaf feeders. However, very little is known about the signals that are involved in defending plants against root feeders and how they are influenced by abiotic factors. We investigated these aspects for the interaction between rice (Oryza sativa) and two root-feeding insects: the generalist cucumber beetle (Diabrotica balteata) and the...

Université de Fribourg

Auxin transport during root gravitropism: transporters and techniques

Geisler, Markus ; Wang, Bangjun ; Zhu, Jinsheng

In: Plant Biology, 2014, vol. 16, no. S1, p. 50-57

Root gravitropism is a complex, plant-specific process allowing roots to grow downward into the soil. Polar auxin transport and redistribution are essential for root gravitropism. Here we summarise our current understanding of underlying molecular mechanisms and involved transporters that establish, maintain and redirect intercellular auxin gradients as the driving force for root gravitropism. We...

Université de Fribourg

Yeast ribosomal protein L40 assembles late into pre-60S ribosomes and is required for their cytoplasmic maturation

Fernandez-Pevida, Antonio ; Rodriguez-Galan, Olga ; Diaz-Quintana, Antonio ; Kressler, Dieter ; Cruz, Jesus de la

In: Journal of Biological Chemistry, 2012, p. -

Most ribosomal proteins play important roles in ribosome biogenesis and function. Herein, we have examined the contribution of the essential ribosomal protein L40 in these processes in the yeast Saccharomyces cerevisiae. Deletion of either the RPL40A or RPL40B gene and in vivo depletion of L40 impair 60S ribosomal subunit biogenesis. Polysome profile analyses reveal the accumulation of half-mers...

Université de Fribourg

Elongation factor 1a mediates the specificity of mitochondrial tRNA import in T. brucei

Bouzaidi-Tiali, Nabile ; Aeby, Eric ; Charrière, Fabien ; Pusnik, Mascha ; Schneider, André

In: The EMBO Journal, 2007, vol. 26, no. 20, p. 4302–4312

Mitochondrial tRNA import is widespread in eukaryotes. Yet, the mechanism that determines its specificity is unknown. Previous in vivo experiments using the tRNAsMet, tRNAIle and tRNALys have suggested that the T-stem nucleotide pair 51:63 is the main localization determinant of tRNAs in Trypanosoma brucei. In the cytosol-specific initiator...