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Université de Neuchâtel

The novel chloroplast outer membrane kinase KOC1 is a required component of the plastid protein import machinery

Zufferey-Arias, Mónica Alexandra ; Kessler, Felix (Dir.)

Thèse de doctorat : Université de Neuchâtel, 2017.

Le chloroplaste est un organite essentiel de la cellule végétale, il est le siège de la photosynthèse. Un événement d’endosymbiose est à l’origine du chloroplaste : une cellule eucaryote primitive a ingéré une cyanobactérie photosynthétique. Pendant l’évolution, la majorité des gènes du chloroplaste primitif ont été transférés vers le noyau. Les protéines issues des...

Université de Neuchâtel

The Novel Chloroplast Outer Membrane Kinase KOC1 Is a Required Component of the Plastid Protein Import Machinery

Zufferey, Mónica ; Montandon, Cyrille ; Douet, Véronique ; Demarsy, Emilie ; Agne, Birgit ; Baginsky, Sacha ; Kessler, Felix

In: Journal of biological chemistry, 2017, vol. 292, no. 17, p. 6952-6964

The biogenesis and maintenance of cell organelles such as mitochondria and chloroplasts require the import of many proteins from the cytosol, a process that is controlled by phosphorylation. In the case of chloroplasts, the import of hundreds of different proteins depends on translocons at the outer and inner chloroplast membrane (TOC and TIC, respectively) complexes. The essential protein TOC159...

Université de Neuchâtel

Role of plastoglobules in metabolite repair in the tocopherol redox cycle

Eugeni Piller, Lucia ; Glauser, Gaétan ; Kessler, Felix ; Besagni, Céline

In: Frontiers in Plant Science, 2014, vol. 5, p. Article 298

Plants are exposed to ever changing light environments and continuously forced to adapt. Excessive light intensity leads to the production of reactive oxygen species that can have deleterious effects on photosystems and thylakoid membranes. To limit damage, plants increase the production of membrane soluble antioxidants such as tocopherols. Here, untargeted lipidomics after high light...

Université de Neuchâtel

Nucleotide binding and dimerization at the chloroplast pre-protein import receptor, atToc33, are not essential in vivo but do increase import efficiency

Aronsson, Henrik ; Combe, Jonathan ; Patel, Ramesh ; Agne, Birgit ; Martin, Meryll ; Kessler, Felix ; Jarvis, Paul

In: The Plant Journal, 2010, vol. 63, no. 2, p. 297-311

The atToc33 protein is one of several pre-protein import receptors in the outer envelope of Arabidopsis chloroplasts. It is a GTPase with motifs characteristic of such proteins, and its loss in the plastid protein import 1 (ppi1) mutant interferes with the import of photosynthesis-related pre-proteins, causing a chlorotic phenotype in mutant plants. To assess the significance of GTPase...

Université de Fribourg

The cuticle and plant defense to pathogens

Metraux, Jean-Pierre ; Serrano, Mario ; Torres, Martha ; Coluccia, Fania ; L’Haridon, Floriane

In: Frontiers in Plant Science, 2014, vol. 5, p. 274

The cuticle provides a physical barrier against water loss and protects against irradiation, xenobiotics and pathogens. Components of the cuticle are perceived by invading fungi and activate developmental processes during pathogenesis. In addition, cuticle alterations of various types induce a syndrome of reactions that often results in resistance to necrotrophs. This article reviews the current...

Université de Fribourg

Pathogen and circadian controlled 1 (PCC1) regulates polar lipid content, ABA-related responses, and pathogen defence in Arabidopsis thaliana

Mir, Ricardo ; Hernández, M. Luisa ; Abou-Mansour, Eliane ; Martínez-Rivas, José Manuel ; Mauch, Félix ; Métraux, Jean-Pierre ; León, José

In: Journal of Experimental Botany, 2013, vol. 64, no. 11, p. 3385–3395

Pathogen and Circadian Controlled 1 (PCC1) was previously characterized as a regulator of defence against pathogens and stress-activated transition to flowering. Plants expressing an RNA interference construct for the PCC1 gene (iPCC1 plants) showed a pleiotropic phenotype. They were hypersensitive to abscisic acid (ABA) as shown by reduced germination potential and seedling establishment, as...

Université de Fribourg

A role for β-sitosterol to stigmasterol conversion in plant–pathogen interactions

Griebel, Thomas ; Zeier, Jürgen

In: The Plant Journal, 2010, vol. 63, no. 2, p. 254–268

Upon inoculation with pathogenic microbes, plants induce an array of metabolic changes that potentially contribute to induced resistance or even enhance susceptibility. When analysing leaf lipid composition during the Arabidopsis thaliana–Pseudomonas syringae interaction, we found that accumulation of the phytosterol stigmasterol is a significant plant metabolic process that occurs upon...

Université de Neuchâtel

Natural Genetic Variation in Arabidopsis: Tools, Traits and Prospects for Evolutionary Ecology

Shindo, Chikako ; Bernasconi, Giorgina ; Hardtke, Christian S.

In: Annals of Botany, 2007, vol. 99, no. 6, p. 1043-1054

Background: The model plant Arabidopsis thaliana (Arabidopsis) shows a wide range of genetic and trait variation among wild accessions. Because of its unparalleled biological and genomic resources, the potential of Arabidopsis for molecular genetic analysis of this natural variation has increased dramatically in recent years. Scope: Advanced genomics has accelerated...

Université de Neuchâtel

Gene targeting in Physcomitrella patens

Schaefer, Didier G.

In: Current Opinion in Plant Biology, 2001, vol. 4, no. 2, p. 143-150

Gene-targeting efficiency in the land plant Physcomitrella patens (Bryophyta) can only be compared with that observed in Saccharomyces cerevisiae. Sequencing programs and microbiological molecular genetic approaches are now being developed to unravel the precise function of plant genes. Physcomitrella patens, as the new ‘green yeast’, might well become a major tool for...