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Université de Fribourg

Characterisation of sunflower-21 (SF21) genes expressed in pollen and pistil of Senecio squalidus (Asteraceae) and their relationship with other members of the SF21 gene family

Allen, Alexandra M. ; Lexer, Christian ; Hiscock, Simon J.

In: Sexual Plant Reproduction, 2010, vol. 23, no. 3, p. 173-186

Two related flower-expressed gene copies belonging to the SF21 (sunflower-21) gene family have been isolated from Senecio squalidus (Oxford Ragwort, Asteraceae). These gene copies are differentially expressed in pollen and pistil tissues; ORSF21B (Oxford Ragwort SF21B) is expressed exclusively in mature pollen, whereas ORSF21A (Oxford Ragwort...

Université de Fribourg

Pollen–pistil interactions and self-incompatibility in the Asteraceae: new insights from studies of Senecio squalidus (Oxford ragwort)

Allen, Alexandra M. ; Thorogood, Christopher J. ; Hegarty, Matthew J. ; Lexer, Christian ; Hiscock, Simon J.

In: Annals of Botany, 2011, p. -

Background: Pollen–pistil interactions are an essential prelude to fertilization in angiosperms and determine compatibility/incompatibility. Pollen–pistil interactions have been studied at a molecular and cellular level in relatively few families. Self- incompatibility (SI) is the best understood pollen–pistil interaction at a molecular level where three different molecular mechanisms...

Université de Fribourg

How sympatric is speciation in the Howea palms of Lord Howe Island?

Babik, Wiesław ; Butlin, Roger K. ; Baker, William J. ; Papadopulos, Alexander S. T. ; Boulesteix, Matthieu ; Anstett, Marie-Charlotte ; Lexer, Christian ; Hutton, Ian ; Savolainen, Vincent

In: Molecular Ecology, 2009, vol. 18, no. 7, p. 3629 - 3638

The two species of the palm genus Howea (Arecaceae) from Lord Howe Island, a minute volcanic island in the Tasman Sea, are now regarded as one of the most compelling examples of sympatric speciation, although this view is still disputed by some authors. Population genetic and ecological data are necessary to provide a more coherent and comprehensive understanding of this emerging model system....

Université de Fribourg

Genetic relationships and variation in reproductive strategies in four closely related bromeliads adapted to neotropical ‘inselbergs’: Alcantarea glaziouana, A. regina, A. geniculata and A. imperialis (Bromeliaceae)

Barbará, Thelma ; Martinelli, Gustavo ; Palma-Silva, Clarisse ; Fay, Michael F. ; Mayo, Simon ; Lexer, Christian

In: Annals of Botany, 2009, vol. 103, no. 1, p. 65-77

Background and Aims: Bromeliads (Bromeliaceae) adapted to rock outcrops or ‘inselbergs’ in neotropical rain forests have been identified as suitable plant models for studying population divergence and speciation during continental plant radiations. Little is known about genetic relationships and variation in reproductive strategies within and among inselberg-adapted species, yet knowledge...

Université de Fribourg

Some like it cold: distribution, ecology and phylogeny of Arenaria bernensis Favarger (Caryophyllaceae) from the western Prealps in Switzerland

Berthouzoz, Marjorie ; Maendly, Simon ; Bétrisey, Sébastien ; Mangili, Sofia ; Prunier, Patrice ; Lexer, Christian ; Kozlowski, Gregor

In: Alpine Botany, 2013, vol. 123, no. 2, p. 65–75

Arenaria bernensis (Caryophyllaceae), traditionally placed within the Arenaria ciliata aggregate and discovered only in 1955, is a poorly studied endemic taxon of western Prealps in Switzerland. In this study, we aimed to deliver the first detailed appraisal on morphology, distribution and ecology of A. bernensis accompanied by first molecular insights into the A. ciliata aggregate using...

Université de Fribourg

Estimating and accounting for genotyping errors in RAD-seq experiments

Bresadola, Luisa ; Link, Vivian ; Buerkle, C. Alex ; Lexer, Christian ; Wegmann, Daniel

In: Molecular Ecology Resources, 2020, vol. 20, no. 4, p. 856–870

In non-model organisms, evolutionary questions are frequently addressed using reduced representation sequencing techniques due to their low cost, ease of use, and because they do not require genomic resources such as a reference genome. However, evidence is accumulating that such techniques may be affected by specific biases, questioning the accuracy of obtained genotypes, and as a...

Université de Fribourg

Admixture mapping in interspecific Populus hybrids identifies classes of genomic architectures for phytochemical, morphological and growth traits

Bresadola, Luisa ; Caseys, Céline ; Castiglione, Stefano ; Buerkle, C. Alex ; Wegmann, Daniel ; Lexer, Christian

In: New Phytologist, 2019, vol. 223, no. 4, p. 2076–2089

The genomic architecture of functionally important traits is key to understanding the maintenance of reproductive barriers and trait differences when divergent populations or species hybridize. We conducted a genome-wide association study (GWAS) to study trait architecture in natural hybrids of two ecologically divergent Populus species. We genotyped 472 seedlings from a natural hybrid zone...

Université de Fribourg

Admixture as the basis for genetic mapping

Buerkle, C. Alex ; Lexer, Christian

In: Trends in Ecology & Evolution, 2008, vol. 23, no. 12, p. 686-694

Genetic mapping in natural populations is increasing rapidly in feasibility and accessibility. As with many areas in genetics, advances in molecular techniques and statistics are drastically altering how we can investigate inheritance in wild organisms. For ecology and evolution, this is particularly significant and promising, because many of the organisms of interest are not amenable to...

Université de Fribourg

Patterns of genetic diversity and differentiation in resistance gene clusters of two hybridizing European Populus species

Casey, Céline ; Stölting, Kai N. ; Barbará, Thelma ; González-Martínez, Santiago C. ; Lexer, Christian

In: Tree Genetics & Genomes, 2015, vol. 11, no. 4, p. 1–12

Resistance genes (R-genes) are essential for long-lived organisms such as forest trees, which are exposed to diverse herbivores and pathogens. In short-lived model species, R-genes have been shown to be involved in species isolation. Here, we studied more than 400 trees from two natural hybrid zones of the European Populus species Populus alba and Populus tremula for microsatellite markers...