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Université de Fribourg

Rapidec Carba NP Test for Rapid Detection of Carbapenemase Producers

Poirel, Laurent ; Nordmann, Patrice

In: Journal of Clinical Microbiology, 2015, vol. 53, no. 9, p. 3003–3008

Performances of the Rapidec Carba NP test (bioMérieux) were evaluated for detection of all types of carbapenemases in Enterobacteriaceae, Acinetobacter baumannii, and Pseudomonas aeruginosa. In less than 2 h after sample preparation, it showed a sensitivity and specificity of 96%. This ready-to-use test is well adapted to the daily need for detection of carbapenemase producers in any laboratory...

Université de Fribourg

Modulation of mgrB gene expression as a source of colistin resistance in Klebsiella oxytoca

Jayol, Aurélie ; Poirel, Laurent ; Villegas, Maria-Virginia ; Nordmann, Patrice

In: International Journal of Antimicrobial Agents, 2015, vol. 46, no. 1, p. 108–110

Gene modifications in the PmrAB and PhoPQ two-component regulatory systems, as well as inactivation of the mgrB gene, are known to be causes of colistin resistance in Klebsiella pneumoniae. The objective of this study was to characterise the mechanism involved in colistin resistance in a Klebsiella oxytoca isolate. A K. oxytoca clinical isolate showing resistance to colistin was recovered in...

Université de Fribourg

Emerging broad-spectrum resistance in Pseudomonas aeruginosa and Acinetobacter baumannii: Mechanisms and epidemiology

Potron, Anaïs ; Poirel, Laurent ; Nordmann, Patrice

In: International Journal of Antimicrobial Agents, 2015, vol. 45, no. 6, p. 568–585

Multidrug resistance is quite common among non-fermenting Gram-negative rods, in particular among clinically relevant species including Pseudomonas aeruginosa and Acinetobacter baumannii. These bacterial species, which are mainly nosocomial pathogens, possess a diversity of resistance mechanisms that may lead to multidrug or even pandrug resistance. Extended-spectrum β-lactamases (ESBLs)...

Université de Fribourg

Clonal distribution of multidrug-resistant Enterobacter cloacae

Girlich, Delphine ; Poirel, Laurent ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2015, vol. 81, no. 4, p. 264–268

A multilocus sequence typing (MLST) scheme including 7 housekeeping genes was used to evaluate whether the current spread of multidrug-resistant Enterobacter cloacae isolates worldwide might be associated to specific successful clones. Fifty E. cloacae clinical isolates of worldwide origin, with various β-lactamase content, and recovered at different periods of time were studied. Forty-four...

Université de Fribourg

Rapid detection of extended-spectrum-β-lactamase-producing Enterobacteriaceae from urine samples by use of the ESBL NDP test

Dortet, Laurent ; Poirel, Laurent ; Nordmann, Patrice

In: Journal of Clinical Microbiology, 2014, vol. 52, no. 10, p. 3701–3706

From June to September 2012, 500 urine samples were recovered from patients with urinary tract infections (UTI) due to Gram-negative bacilli (≥10⁴ leukocytes/ml and ≥10⁵ Gram-negative isolates/ml) who visited the University hospital Bicêtre (France). They were challenged with extended-spectrum-β-lactamase (ESBL)-producing Enterobacteriaceae (ESBL-E) using the rapid diagnostic ESBL NDP...

Université de Fribourg

Resistance to colistin associated with a single amino acid change in protein PmrB among Klebsiella pneumoniae isolates of worldwide Origin

Jayol, Aurélie ; Poirel, Laurent ; Brink, Adrian ; Villegas, Maria-Virginia ; Yilmaz, Mesut ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2014, vol. 58, no. 8, p. 4762–4766

A series of colistin-resistant Klebsiella pneumoniae isolates recovered from different countries was investigated in order to evaluate the involvement of the PmrA/PmrB two-component system in this resistance. Six isolates possessed a mutated PmrB protein, which is encoded by the pmrB gene, part of the pmrCAB operon involved in lipopolysaccharide modification. The same amino acid substitution...

Université de Fribourg

Multidrug-resistant Acinetobacter baumannii strains carrying the blaOxA-23 and the blaGES-11 genes in a neonatology center in Tunisia

Charfi-Kessis, Karama ; Mansour, Wejdene ; Khalifa, Anis Ben Haj ; Mastouri, Maha ; Nordmann, Patrice ; Aouni, Mahjoub ; Poirel, Laurent

In: Microbial Pathogenesis, 2014, vol. 74, p. 20–24

Multidrug-resistant and difficult-to-treat Acinetobacter baumannii may be responsible for nosocomial infections. The production of carbapenem-hydrolyzing class D β-lactamases (CHDLs) and extended-spectrum β-lactamase (ESBLs) of the GES type possessing a carbapenemase activity has been increasingly reported worldwide in A. baumannii. The aim of this study was to analyze the...

Université de Fribourg

The difficult-to-control spread of carbapenemase producers in Enterobacteriaceae worldwide

Nordmann, Patrice ; Poirel, Laurent

In: Clinical Microbiology and Infection, 2014, p. -

Spread of carbapenemase producers in Enterobacteriaceae is now identified worldwide. Three main carbapenemases are reported which belong to three classes of ß-lactamases that are KPC, NDM and OXA-48. The main reservoirs of KPC are Klebsiella pneumoniae in the USA, Israel, Greece and Italy, of NDM are K. pneumoniae and Escherichia coli in the Indian subcontinent, and of OXA-48 are K. pneumoniae...

Université de Fribourg

Characteristics of Escherichia coli sequence type 131 isolates that produce extended-spectrum β-lactamases: global distribution of the H30-Rx sublineage

Peirano, Gisele ; Bij, Akke K. van der ; Freeman, Joshua L. ; Poirel, Laurent ; Nordmann, Patrice ; Costello, Michael ; Tchesnokova, Veronika L. ; Pitout, Johann D. D.

In: Antimicrobial Agents and Chemotherapy, 2014, vol. 58, no. 7, p. 3762–3767

We designed a study to describe the characteristics of sequence type 131 (ST131) lineages, including the H30-Rx sublineage, among a global collection of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates from 9 countries collected from 2000 to 2011. A total of 240 nonrepeat isolates from Canada, the United States, Brazil, the Netherlands, France, the United Arab...