Université de Fribourg

Rapid detection of carbapenemase-producing Pseudomonas spp. using the NitroSpeed-Carba NP test

Sadek, Mustafa ; Poirel, Laurent ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2021, vol. 99, no. 3, p. 115280

The NitroSpeed-Carba NP test was used to rapidly detect and discriminate between the different types of carbapenemases (classes A, B, and D) within 30 minutes among a collection of 202 Pseudomonas sp. strains (mostly Pseudomonas aeruginosa). A total of 99 carbapenemase–(including enzymes exhibiting weak carbapenemase activity such as several Guyana Extended-Spectrum (GES)-ß-lactamases) and...

Université de Fribourg

Cross-border emergence of Escherichia coli producing the carbapenemase NDM-5 in Switzerland and Germany

Chakraborty, Trinad ; Sadek, Mustafa ; Yao, Yancheng ; Imirzalioglu, Can ; Stephan, Roger ; Poirel, Laurent ; Nordmann, Patrice

In: Journal of Clinical Microbiology, 2020, p. -

A series of clinical NDM-5-producing E. coli isolates obtained from two surveillance networks of carbapenem-producing Enterobacterales from 2018-2019, namely Switzerland (NARA) and Germany (SurvCARE), were analyzed. The 33 NDM-5- producing E. coli isolates were highly resistant to β-lactams including the novel ß- lactam/ß-lactamase inhibitor combinations (ceftazidime/avibactam, ...

Université de Fribourg

Genetic features leading to reduced susceptibility to aztreonam-avibactam among metallo-β-lactamase-producing escherichia coli isolates

Sadek, Mustafa ; Juhas, Mario ; Poirel, Laurent ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2020, vol. 64, no. 2020-12, p. -

Metallo-β-lactamase (MBL)-producing Escherichia coli isolates resistant to the newly developed β-lactam/β-lactamase inhibitor drug combination aztreonam-avibactam (ATM-AVI) have been reported. Here, we analyzed a series of 118 clinical MBL- producing E. coli isolates of various geographical origins for susceptibility to ATM-AVI. The nature of the PBP3 protein sequence and the occurrence of...

Université de Fribourg

Fast and reliable detection of carbapenemase genes in various Gram negatives using a new commercially available fluorescence-based real-time polymerase chain reaction platform

Sadek, Mustafa ; Demord, Anthony ; Poirel, Laurent ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2020, vol. 98, no. 3, p. 115127

The Revogene® Carba C assay is a real-time polymerase chain reaction–based assay that runs on the microfluidic Revogene platform. It was recently designed for the detection of genes encoding the 5 major carbapenemases (NDM, VIM, IMP, KPC, and OXA-48) from various Gram-negatives. A total of 145 clinical Gram-negative strains (96 carbapenemase producers and 49 non–carbapenemase producers)...

Université de Fribourg

First genomic characterization of blavim-1 and mcr-9-coharbouring enterobacter hormaechei isolated from food of animal origin

Sadek, Mustafa ; Nariya, Hirofumi ; Shimamoto, Toshi ; Kayama, Shizuo ; Yu, Liansheng ; Hisatsune, Junzo ; Sugai, Motoyuki ; Nordmann, Patrice ; Poirel, Laurent ; Shimamoto, Tadashi

In: Pathogens, 2020, vol. 9, no. 9, p. 687

We describe here the complete genome sequence of an Enterobacter hormaechei ST279 coharbouring blaVIM-1 and mcr-9 recovered from uncooked beef patty in June 2017, Egypt. The tested isolate was resistant to carbapenem but susceptible to colistin (minimum inhibitory concentration (MIC), 0.5 μg/mL). The antimicrobial susceptibility profile and conjugation experiments were performed. The entire...

Université de Fribourg

Draft genome sequence of a blaNDM-1- and blaOXA-244-carrying multidrug-resistant Escherichia coli D-ST69 clinical isolate from Egypt

Soliman, Ahmed M. ; Ramadan, Hazem ; Sadek, Mustafa ; Nariya, Hirofumi ; Shimamoto, Toshi ; Hiott, Lari M. ; Frye, Jonathan G. ; Jackson, Charlene R. ; Shimamoto, Tadashi

In: Journal of Global Antimicrobial Resistance, 2020, vol. 22, p. 832–834

Objectives: This study describes the first draft genome sequence of a multidrug- resistant (MDR) Escherichia coli D-ST69 clinical isolate from Egypt carrying blaNDM-1 and blaOXA-244.Methods: The strain was isolated in December 2014 from a wound pus swab of a male patient in the city of Kafr El-Sheikh using MacConkey agar containing 2 μg/mL meropenem. The strain was subjected to antimicrobial ...

Université de Fribourg

A selective culture medium for screening ceftazidime-avibactam resistance in Enterobacterales and Pseudomonas aeruginosa

Sadek, Mustafa ; Poirel, Laurent ; Tinguely, Camille ; Nordmann, Patrice

In: Journal of Clinical Microbiology, 2020, vol. 58, no. 9, p. -

The SuperCAZ/AVI medium was developed for screening ceftazidime-avibactam (CZA) resistance among Gram-negative bacteria (Enterobacterales and Pseudomonas aeruginosa). It was evaluated using 50 CZA-susceptible and 42 CZA- resistant Gram-negative isolates. Its sensitivity and specificity of detection were 100%. Excellent performance of the medium was also observed by testing spiked stools, with ...

Université de Fribourg

NitroSpeed-carba NP test for rapid detection and differentiation between different classes of carbapenemases in Enterobacterales

Nordmann, Patrice ; Sadek, Mustafa ; Demord, Anthony ; Poirel, Laurent

In: Journal of Clinical Microbiology, 2020, vol. 58, no. 9, p. -

A biochemical test (NitroSpeed-Carba NP test) was developed to identify carbapenemase production in Enterobacterales and to discriminate between the different types of clinically significant carbapenemases (Ambler classes A, B, and D). It is based on two main features, namely, the hydrolysis by all β-lactamases, including carbapenemases of the nitrocefin substrate, and the capacity of...

Université de Fribourg

Rapid polymyxin/pseudomonas NP test for rapid detection of polymyxin susceptibility/resistance in Pseudomonas aeruginosa

Sadek, Mustafa ; Tinguely, Camille ; Poirel, Laurent ; Nordmann, Patrice

In: European Journal of Clinical Microbiology & Infectious Diseases, 2020, p. -

Polymyxins are becoming an added value for treating infections due to multidrug-resistant Pseudomonas aeruginosa. Therefore, the Rapid Polymyxin/Pseudomonas NP test was developed for the identification of polymyxin resistance in P. aeruginosa isolates. This test detects the bacterial growth in a medium containing a defined concentration of colistin. Formation of basic metabolites is visually...