(Strept)avidin as host for biotinylated coordination complexes: stability, chiral discrimination, and cooperativity
- Loosli, Andreas Institute of Chemistry, University of Neuchâtel, Switzerland
- Rusbandi, Untung Edy Institute of Chemistry, University of Neuchâtel, Switzerland
- Gradinaru, Julieta Institute of Chemistry, University of Neuchâtel, Switzerland
- Bernauer, Klaus Institute of Chemistry, University of Neuchâtel, Switzerland
- Schläpfer, Carl-Wilhelm Institute of Inorganic Chemistry, University of Fribourg, Switzerland
- Meyer, Michel Laboratoire d'Ingénierie Moléculaire pour la Séparation et les Applications des Gaz, Université de Bourgogne, Faculté des Sciences, France
- Mazurek, Sylwester Institute of Chemistry, University of Neuchâtel, Switzerland - Laboratory of Chemometrics, National Institute of Chemistry, Ljubljana, Slovenia
- Novic, Marjana Laboratory of Chemometrics, National Institute of Chemistry, Ljubljana, Slovenia
- Ward, Thomas R. Institute of Chemistry, University of Neuchâtel, Switzerland
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24.12.2005
Published in:
- Inorganic Chemistry. - 2006, vol. 45, no. 2, p. 660-668
English
Incorporation of a biotinylated ruthenium tris(bipyridine) [Ru(bpy)₂(Biot-bpy)]²⁺ (1) in either avidin or streptavidin-(strept)avidin-can be conveniently followed by circular dichroism spectroscopy. To determine the stepwise association constants, cooperativity, and chiral discrimination properties, diastereopure (Λ and δ)-1 species were synthesized and incorporated in tetrameric (strept)avidin to afford (δ-[Ru(bpy)₂(Biot-bpy)]²⁺)x⊂avidin, (Λ- [Ru(bpy)₂(Biot-bpy)]²⁺)x⊂avidin, (δ-[Ru(bpy)₂(Biot- bpy)]²⁺)x⊂streptavidin, and (Λ-[Ru(bpy)₂(Biot-bpy)]²⁺) x⊂streptavidin (x = 1-4) For these four systems, the overall stability constants are log β₄ = 28.6, 30.3, 36.2, and 36.4, respectively. Critical analysis of the CD titrations data suggests a strong cooperativity between the first and the second binding event (x = 1, 2) and a pronounced difference in affinity between avidin and streptavidin for the dicationic guest 1 as well as modest enantiodiscrimination properties with avidin as host.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Chimie
- Language
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- English
- Classification
- Chemistry
- License
- License undefined
- Identifiers
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- RERO DOC 8655
- DOI 10.1021/ic051405t
- Persistent URL
- https://folia.unifr.ch/unifr/documents/300492
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