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Staphylococcal biofilm formation on the surface of three different calcium phosphate bone grafts: a qualitative and quantitative in vivo analysis

Furustrand Tafin, Ulrika ; Betrisey, Bertrand ; Bohner, Marc ; Ilchmann, Thomas ; Trampuz, Andrej ; Clauss, Martin

In: Journal of Materials Science: Materials in Medicine, 2015, vol. 26, no. 3, p. 1-8

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    Titel
    • Staphylococcal biofilm formation on the surface of three different calcium phosphate bone grafts: a qualitative and quantitative in vivo analysis
    Autor
    • Furustrand Tafin, Ulrika. Infectious Diseases Service, Department of Internal Medicine, University Hospital Lausanne (CHUV), Lausanne, Switzerland
    • Betrisey, Bertrand. Infectious Diseases Service, Department of Internal Medicine, University Hospital Lausanne (CHUV), Lausanne, Switzerland
    • Bohner, Marc. RMS Foundation, Bettlach, Switzerland
    • Ilchmann, Thomas. Department for Orthopaedics and Trauma Surgery, Clinic for Orthopaedics and Trauma Surgery, Kantonsspital Baselland Liestal, Rheinstreet 26, 4410, Liestal, Switzerland
    • Trampuz, Andrej. Infectious Diseases Service, Department of Internal Medicine, University Hospital Lausanne (CHUV), Lausanne, Switzerland
    • Clauss, Martin. Infectious Diseases Service, Department of Internal Medicine, University Hospital Lausanne (CHUV), Lausanne, Switzerland
    Dokumententyp
    • Postprint
    Sprache
    • Englisch
    Veröffentlicht in
    • Journal of Materials Science: Materials in Medicine, 2015, vol. 26, no. 3, p. 1-8. Springer US; http://www.springer-ny.com
    Andere elektronische Ausgabe
    Klassifikation
    • Gesundheit
    OAI-PMH-ID
    • oai:doc.rero.ch:332889
    Summary
    Differences in physico-chemical characteristics of bone grafts to fill bone defects have been demonstrated to influence in vitro bacterial biofilm formation. Aim of the study was to investigate in vivo staphylococcal biofilm formation on different calcium phosphate bone substitutes. A foreign-body guinea-pig infection model was used. Teflon cages prefilled with β-tricalcium phosphate, calcium-deficient hydroxyapatite, or dicalcium phosphate (DCP) scaffold were implanted subcutaneously. Scaffolds were infected with 2×103 colony-forming unit of Staphylococcus aureus (two strains) or S. epidermidis and explanted after 3, 24 or 72h of biofilm formation. Quantitative and qualitative biofilm analysis was performed by sonication followed by viable counts, and microcalorimetry, respectively. Independently of the material, S. aureus formed increasing amounts of biofilm on the surface of all scaffolds over time as determined by both methods. For S. epidermidis, the biofilm amount decreased over time, and no biofilm was detected by microcalorimetry on the DCP scaffolds after 72h of infection. However, when using a higher S. epidermidis inoculum, increasing amounts of biofilm were formed on all scaffolds as determined by microcalorimetry. No significant variation in staphylococcal in vivo biofilm formation was observed between the different materials tested. This study highlights the importance of in vivo studies, in addition to in vitro studies, when investigating biofilm formation of bone grafts.