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Reactivity of hexanuclear ruthenium metallaprisms towards nucleotides and a DNA decamer

Paul, Lydia ; Therrien, Bruno ; Furrer, Julien

In: JBIC Journal of Biological Inorganic Chemistry, 2015, vol. 20, no. 1, p. 49-59

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    Titre
    • Reactivity of hexanuclear ruthenium metallaprisms towards nucleotides and a DNA decamer
    Auteur
    • Paul, Lydia. Departement für Chemie und Biochemie, Universität Bern, 3012, Bern, Switzerland
    • Therrien, Bruno. Institut de Chimie, Université de Neuchâtel, 2000, Neuchâtel, Switzerland
    • Furrer, Julien. Departement für Chemie und Biochemie, Universität Bern, 3012, Bern, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • JBIC Journal of Biological Inorganic Chemistry, 2015, vol. 20, no. 1, p. 49-59. Springer Berlin Heidelberg
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:332520
    Summary
    The reactivity of three hexacationic arene ruthenium metallaprisms towards isolated nucleotides and a short DNA strand was investigated using NMR spectroscopy, ESI mass spectrometry, UV/Vis and circular dichroism spectroscopy. The metallaprism built from oxalato-bridging ligands reacts rapidly in the presence of deoxyguanosine monophosphate (dGMP) and deoxyadenosine monophosphate, while the benzoquinonato derivative only reacts with dGMP. On the other hand, the larger metallaprism incorporating naphtoquinonato bridges remains stable in the presence of nucleotides. The reactivity of the three hexacationic metallaprisms with the decameric oligonucleotide d(CGCGATCGCG)2 was also investigated. Analysis of the NMR, MS, UV/Vis and CD data suggests that no adducts are formed between the oligonucleotide and the metallaprisms, but electrostatic interactions, leading to partial unwinding of the double-stranded oligonucleotide, were evidenced.